TFEC antibody (Internal Region)

Catalog No. ABIN372800

The Goat Polyclonal anti-TFEC antibody is suitable to detect TFEC in samples from Mouse and Rat. It has been validated for IHC (p) and EIA.

Quick Overview for TFEC antibody (Internal Region) (ABIN372800)

Target: TFEC (Transcription Factor EC (TFEC))

Reactivity: Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This TFEC antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-TFEC Antibody

Binding Specificity: Internal Region

Specificity: Recognizes Transcription Factor EC (TFEC).

Cross-Reactivity (Details): Species reactivity (expected):Rat.
Species reactivity (tested):Mouse.

Purification: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide (C-HITKQQTHPERN) from an Internal region of Mouse TFEC.

Isotype: IgG

Application Details

Application Notes: ELISA: 1/32000. Immunohistochemistry on Paraffin Sections: 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: Tris saline buffer, pH 7.3 containing 0.5 % BSA as stabilizer and 0.02 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for TFEC

Target: TFEC (Transcription Factor EC (TFEC))

Alternative Name: TFEC

Background: The DNA-binding factor TFE3 contains adjacent helix-loop-helix (HLH) and leucine zipper (LZ) domains flanked by an upstream basic region. These protein motifs are frequently observed in other transcription factors and are particularly common to members of the Myc family. TFE3 is ubiquitously expressed and can directly associates with DNA as either homodimers or heterodimers formed with two related proteins, TFEB or TFEC. TFE3 binds to and activates the microE3 motif of the immunoglobulin heavy-chain enhancer to induce B-cell-specific gene transcription and DNA recombination. TFEB binds to the major late promoter of adenovirus and specifically associates with DNA as both a homodimer and a heterodimer with TFE3. TFEB is expressed at low levels in the embryo but at high levels in the trophoblast cells of the placenta, where it plays a critical role in regulating normal vascularization of the placenta. TFEC shares a bHLH/LZ structure with TFE3 and a closely related protein microphthalmia-associated transcription factor (MITF), which is critically involved in melanocyte differentiation. Unlike TFE3, the expression of TFEC is largely restricted to fibroblasts, myoblasts, chondrosarcoma cells, and myeloma cells.Synonyms: TCFEC, TFE-C, TFEC-L, TFECL, Transcription factor EC, Transcription factor EC-like

Gene ID: 21426

NCBI Accession: NP_112475

UniProt: Q9WTW4