TMPRSS5 antibody (C-Term)

Catalog No. ABIN374177

The Goat Polyclonal anti-TMPRSS5 antibody is suitable to detect TMPRSS5 in samples from Human, Mouse and Rat. It has been validated for WB.

Quick Overview for TMPRSS5 antibody (C-Term) (ABIN374177)

Target: TMPRSS5 (Transmembrane Protease, serine 5 (TMPRSS5))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This TMPRSS5 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TMPRSS5 Antibody

Binding Specificity: C-Term

Sequence: C-LDWIHDTAQD SLL

Specificity: This antibody detects TMPRSS5 at C-term.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_110397.2 Genename: TMPRSS5

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.5-1.5 μg/mL. Experiments gave bands at approx 50 kDa and 20 kDa inHuman Brain (Cerebral Cortex) lysates and in Mouse and Rat Brain lysates after 0.5 μg/mLantibody staining. These bands correspond to earlier findings in literature with differentantibodies (Yamaguchi et al, J Biol Chem.2002 Mar 1,277(9): 6806-12, PMID: 11741986). This protein has a calculated MW of 49.0 kDa according to NP_110397.1.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for TMPRSS5

Target: TMPRSS5 (Transmembrane Protease, serine 5 (TMPRSS5))

Alternative Name: TMPRSS5 / Spinesin

Background: Synonyms: MGC141886, MGC148044, Transmembrane protease serine 5

Gene ID: 80975

NCBI Accession: NP_110397

UniProt: Q9H3S3