SF3B4 antibody (N-Term)

Catalog No. ABIN374245

This Goat Polyclonal antibody specifically detects SF3B4 in WB. It exhibits reactivity toward Human, Mouse, Cow, Dog and Pig.

Quick Overview for SF3B4 antibody (N-Term) (ABIN374245)

Target: SF3B4 (Splicing Factor 3b, Subunit 4, 49kDa (SF3B4))

Reactivity: Human, Mouse, Cow, Dog, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This SF3B4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SF3B4 Antibody

Binding Specificity: N-Term

Sequence: AAGPISERNQ DAT-C

Specificity: This antibody detects SF3B4 / SAP49 at N-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Dog, Pig, CowSpecies reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from N Terminus of the protein sequence according to NP_005841.1 Genename: SF3B4

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.1-0.3 μg/mL. Approx 48 kDa band observed in Human HeLa lysates(calculated MW of 44.8 kDa according to NP005841.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for SF3B4

Target: SF3B4 (Splicing Factor 3b, Subunit 4, 49kDa (SF3B4))

Alternative Name: SF3B4 / SAP49

Background: Synonyms: Pre-mRNA-splicing factor SF3b 49 kDa subunit, SAP 49, SAP-49, SF3b50, Spliceosome-associated protein 49, Splicing factor 3B subunit 4

Gene ID: 10262

NCBI Accession: NP_005841

UniProt: Q15427