UBE2K antibody (C-Term)

Catalog No. ABIN374290

This Goat Polyclonal antibody specifically detects UBE2K in WB. It exhibits reactivity toward Human, Mouse, Dog, Cow and Pig.

Quick Overview for UBE2K antibody (C-Term) (ABIN374290)

Target: UBE2K (Ubiquitin-Conjugating Enzyme E2K (UBE2K))

Reactivity: Human, Mouse, Dog, Cow, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This UBE2K antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-UBE2K Antibody

Binding Specificity: C-Term

Sequence: C-SWDVETATEL LLSN

Specificity: This antibody recognizes UBE2K/HIP2 (C-term). It is expected to recognise all reported isoforms.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Canine (Dog), Pig and Cow.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_005330.1, NP_001104582.1, NP_001104583.1. Genename: UBE2K

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/2000. Western blot: 1-3 μg/mL. Approx 24 kDa band observed in Jurkat cell lysates (CalculatedMW of 22.4 kDa according to NP_005330).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted at (in aliquots) at -20 °C.

Target Details for UBE2K

Target: UBE2K (Ubiquitin-Conjugating Enzyme E2K (UBE2K))

Alternative Name: HIP2 / UBE2K

Background: The protein encoded by this gene belongs to the ubiquitin-conjugating enzyme family. This protein interacts with RING finger proteins, and it can ubiquitinate huntingtin, the gene product for Huntington's disease. Known functions for this protein include a role in aggregate formation of expanded polyglutamine proteins and the suppression of apoptosis in polyglutamine diseases, a role in the dislocation of newly synthesized MHC class I heavy chains from the endoplasmic reticulum, and involvement in foam cell formation. Multiple transcript variants encoding different isoforms have been identified for this gene. HIP2 belongs to the ubiquitin conjugating enzyme family. It binds selectively to a large region at the N terminus of huntingtin. This interaction is not influenced by the length of the huntingtin polyglutamine tract. This protein has been implicated in the degradation of huntingtin and suppression of apoptosis. Catalyzes the covalent attachment of ubiquitin to other proteins. Mediates the selective degradation of short lived and abnormal proteins. Ubiquitinates huntingtin. May mediate foam cell formation by the suppression of apoptosis of lipid bearing macrophages through ubiquitination and subsequent degradation of p53.Synonyms: E2(25K), E2-25K, HIP-2, Huntingtin-interacting protein 2, LIG, Ubiquitin carrier protein, Ubiquitin-conjugating enzyme E2 K, Ubiquitin-protein ligase

Gene ID: 3093

NCBI Accession: NP_005330

UniProt: P61086