TCP1 alpha/CCTA antibody (C-Term)

Catalog No. ABIN374406

The Goat Polyclonal anti-TCP1 alpha/CCTA antibody (ABIN374406) specifically detects TCP1 alpha/CCTA in WB, IHC (p) and EIA. The antibody is reactive with Human samples.

Quick Overview for TCP1 alpha/CCTA antibody (C-Term) (ABIN374406)

Target: TCP1 alpha/CCTA (TCP1) (T-Complex 1 (TCP1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This TCP1 alpha/CCTA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-TCP1 alpha/CCTA Antibody

Binding Specificity: C-Term

Sequence: C-SYEDAVHSGA LND

Specificity: This antibody detects CCT1 / TCP1 at C-term. It is expected to recognise both reported isoforms.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_110379.2, NP_001008897.1. Genename: TCP1

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1: 32000. Western blot: 0.5-2 μg/mL. Approx 60 kDa band observed in Hela, Human Lung and HumanTestis lysates (predicted MW of 60 kDa according to NP_110379.2). Immunohistochemistry: 1-2 μg/mL. In paraffin embedded Human Testis shows onlycytoplasmic staining in some germ cells, and both cytoplasmic and nuclear staining inother germ cells with unstained nucleoli.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for TCP1 alpha/CCTA

Target: TCP1 alpha/CCTA (TCP1) (T-Complex 1 (TCP1))

Alternative Name: CCT1 / TCP1

Background: TCP1 alpha is a ~60 kDa protein constitutively expressed in almost all eukaryotic cells, and is upregulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone that is known to be involved in the folding of actin and tubulin. The family of proteins termed chaperonins act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly, and share many characteristics with Heat Shock Protein 70 (HSP 70) including high abundance, induction by environmental stress, and ATPase activity. The chaperonin family includes the mitochondrial HSP60, Escherichia coli GroEL, the plastid Rubisco subunit binding protein, and the archaebacterial protein TF55. The TCP1 sequence shows nearly 40 % identity to TF55, but only minimal similarity to HSP60 and GroEL.Synonyms: CCT alpha, CCTA, Chaperonin containing T complex polypeptide 1 subunit 1, T-complex protein 1 subunit alpha, TCP-1-alpha, TCP1 alpha

Gene ID: 6950

NCBI Accession: NP_110379

UniProt: P17987