FTCD antibody (Internal Region)

Catalog No. ABIN374454

The Goat Polyclonal anti-FTCD antibody (ABIN374454) specifically detects FTCD in WB, IHC (p) and EIA. The antibody is reactive with Human, Mouse, Rat and Pig samples.

Quick Overview for FTCD antibody (Internal Region) (ABIN374454)

Target: FTCD (Formiminotransferase Cyclodeaminase (FTCD))

Reactivity: Human, Mouse, Rat, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This FTCD antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-FTCD Antibody

Binding Specificity: Internal Region

Sequence: CLREQGRGKD QPGRL

Specificity: This antibody recognizes 58KGolgi protein (Internal).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat and Pig.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from the internal region of the protein sequence according to NP_006648.1, NP_996848.1 Genename: FTCD

Application Details

Application Notes: Peptide ELISA: Antibody Detection Limit: 1/64000. Western blot: 0.01-0.03 μg/mL. Detects a approx. 60 kDa band in Human Liver lysates (calculatedMW of 58.0 kDa according to NP_006648.1 and NP_996848.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for FTCD

Target: FTCD (Formiminotransferase Cyclodeaminase (FTCD))

Alternative Name: 58K Golgi Protein

Background: 58K Golgi protein antibodies are excellent for use as markers for the Golgi complex. The 58K Golgi protein has been identified as being FTCD, a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool. Defects in FTCD are the cause of glutamate formiminotransferase deficiency, also known as formiminoglutamicaciduria (FIGLU-uria). It is an autosomal recessive disorder. Features of a severe phenotype, include elevated levels of formiminoglutamate (FIGLU) in the urine in response to histidine administration, megaloblastic anemia, and mental retardation. Features of a mild phenotype include high urinary excretion of FIGLU in the absence of histidine administration, mild developmental delay, and no hematological abnormalities.Synonyms: FTCD, Formimidoyltransferase-cyclodeaminase, Formiminotransferase-cyclodeaminase, Golgi Marker 58K, Golgi marker, LCHC1

Gene ID: 10841, 9606

UniProt: O95954