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Caspase 4 antibody (Caspase 4, Apoptosis-Related Cysteine Peptidase) (Internal Region)

Details for Product anti-CASP4 Antibody No. ABIN374466, Supplier: Login to see
Antigen
  • CASP4
  • ICE(rel)II
  • ICEREL-II
  • ICH-2
  • Mih1/TX
  • TX
  • Casp11
  • Caspl
  • ich-3
  • CASP1
  • CASP13
Alternatives
anti-Human Caspase 4 antibody for Immunohistochemistry (Paraffin-embedded Sections)
Epitope
Internal Region
42
26
14
10
6
6
5
5
4
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
168
75
32
20
3
1
1
Host
Goat
184
22
15
13
7
Clonality
Polyclonal
Conjugate
This Caspase 4 antibody is un-conjugated
13
11
3
2
2
2
2
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
197
88
52
45
12
12
12
8
8
6
3
3
2
1
1
Supplier
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Immunogen Peptide from the internal region of the protein sequence according to NP_001216.1, NP_150649.1.
Sequence CKERAEEIYP IKERN
Specificity This antibody is expected to recognise both isoforms of CASP4 (alpha, represented by NP_001216.1 and gamma, NP-150649.1).
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Ammonium sulphate precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
Alternative Name Caspase-4 (CASP4 Antibody Abstract)
Background Caspase 4 is a 377 amino acid protein (43 kDa) that belongs to the Ced3/ICE family of cystein proteases. Caspase 4 is an "upstream" caspase with a long prodomain. It undergoes a cleavage upon activation, leading to p20 and p10 subunits which have 67 % homology to mature Caspase 1 over the equivalent coding sequences. Caspase 4 is capable of cleaving itself and the p30 Caspase 1 precursor, and of inducing apoptosis in transfected insect or COS cells. In high concentrations, it can also cleave the nuclear DNA enzyme PARP but the biological relevance of this cleavage is unclear. Caspase 4 mRNA is found in most tissues examined with the exception of the brain. Highest expression is found in spleen and lung, with moderate expression in heart and liver. Low expression is observed with skeletal muscle, kidney, and testis.Synonyms: CASP4, ICE(rel)-II, ICH2, Protease ICH-2, Protease TX
Gene ID 837, 9606
UniProt P49662
Research Area Apoptosis/Necrosis
Pathways Apoptosis, Caspase Cascade in Apoptosis
Application Notes Peptide ELISA: 1/16000. Western blot: 0.3-1.0 μg/mL. Approx 40 kDa band observed in Human Heart lysates(calculated MW of 43.0 kDa according to NP_001216.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.
Supplier Images
 image for anti-Caspase 4 antibody (Caspase 4, Apoptosis-Related Cysteine Peptidase) (Internal Region) (ABIN374466) anti-Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4) (Internal Region) antibody
Background publications Munday, Vaillancourt, Ali et al.: "Molecular cloning and pro-apoptotic activity of ICErelII and ICErelIII, members of the ICE/CED-3 family of cysteine proteases." in: The Journal of biological chemistry, Vol. 270, Issue 26, pp. 15870-6, 1995 (PubMed).