Caspase 4 antibody (Internal Region)

Catalog No. ABIN185284

This Goat Polyclonal antibody specifically detects Caspase 4 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for Caspase 4 antibody (Internal Region) (ABIN185284)

Target: Caspase 4 (CASP4) (Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Caspase 4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-Caspase 4 Antibody

Binding Specificity: Internal Region

Purpose: CASP4

Sequence: CKERAEEIYP IKERN

Specificity: This antibody is expected to recognise both isoforms of CASP4 (alpha, represented by NP_001216.1 and gamma, NP_150649.1).

Cross-Reactivity: Cat, Cow, Dog, Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence CKERAEEIYPIKERN, from the internal region of the protein sequence according to NP_001216.1, NP_150649.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 40 kDa band observed in Human Heart lysates (calculated MW of 43.3 kDa according to NP_001216.1). Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Caspase 4

Target: Caspase 4 (CASP4) (Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4))

Alternative Name: CASP4

Background: CASP4, TX, ICH-2, Mih1/TX, ICEREL-II, ICE(rel)II, caspase 4, apoptosis-related cysteine protease, apoptotic cysteine protease Mih1/TX, caspase 4

Gene ID: 837

NCBI Accession: NP_001216, NP_150649

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity