Caspase 4 antibody

Catalog No. ABIN2745522

The Rat Monoclonal anti-Caspase 4 antibody is suitable to detect Caspase 4 in samples from Mouse and Rat. It has been validated for WB, ELISA, IP and FACS.

Quick Overview for Caspase 4 antibody (ABIN2745522)

Target: Caspase 4 (CASP4) (Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4))

Reactivity: Mouse, Rat

Host: Rat

Clonality: Monoclonal

Conjugate: This Caspase 4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS)

Clone: 8A5

Product Details anti-Caspase 4 Antibody

Purpose: anti-Caspase-11 (mouse), mAb (8A5)

Specificity: Recognizes mouse and rat full-length caspase-11 protein. Detects two bands of ~38 kDa and ~43 kDa by Western blot.

Characteristics: Monoclonal Antibody. Recognizes mouse and rat full-length caspase-11 protein. Detects two bands of ~38 kDa and ~43 kDa by Western blot. Isotype: Rat IgG1kappa. Clone: 8A5. Applications: ELISA, FACS, IP, WB. Liquid. In PBS containing 0.02 % sodium azide. Caspase-11 not only activates caspase-1, that is required for the maturation of proinflammatory cytokines such as interleukin-1 (IL-1) and interleukin-18 (IL-18), but also activates caspase-3, leading to cellular apoptosis under pathological conditions. In most cells, caspase-11 is only expressed upon induction with pro-inflammatory stimuli. Caspase-11 is an essential mediator of endotoxic shock and caspase-11-deficient mice are resistant to endotoxic shock. LPS-induced caspase-11 regulates lymphocyte apoptosis by activating both caspase-3 and caspase-7.

Purity: >95 % (SDS-PAGE)

Endotoxin Level: <0.1EU/μg purified protein (LAL test, Lonza).

Immunogen: Recombinant mouse caspase-11 (p20 fragment).

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: In PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: After opening, prepare aliquots and store at -20 °C.Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment:

+4°C

-20°C

Stable for at least 1 year after receipt when stored at -20°C.

Expiry Date: 12 months

Target Details for Caspase 4

Target: Caspase 4 (CASP4) (Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4))

Alternative Name: Caspase-11

Background:

Alternate Names/Synonyms: Caspase-11 (mouse), Protease ICH-3 (mouse)

Product Description: Caspase-11 not only activates caspase-1, that is required for the maturation of proinflammatory cytokines such as interleukin-1 (IL-1) and interleukin-18 (IL-18), but also activates caspase-3, leading to cellular apoptosis under pathological conditions. In most cells, caspase-11 is only expressed upon induction with pro-inflammatory stimuli. Caspase-11 is an essential mediator of endotoxic shock and caspase-11-deficient mice are resistant to endotoxic shock. LPS-induced caspase-11 regulates lymphocyte apoptosis by activating both caspase-3 and caspase-7.

UniProt: P70343

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity