Caspase 4 antibody (AA 78-265)

Catalog No. ABIN7434005

The Rabbit Polyclonal anti-Caspase 4 antibody is suitable to detect Caspase 4 in samples from Human. It has been validated for WB, ELISA, ICC, IHC (p) and IHC (fro).

Quick Overview for Caspase 4 antibody (AA 78-265) (ABIN7434005)

Target: Caspase 4 (CASP4) (Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Caspase 4 Antibody

Binding Specificity: AA 78-265

Purpose: Polyclonal Antibody to Caspase 4 (CASP4)

Specificity: The antibody is a rabbit polyclonal antibody raised against CASP4. It has been selected for its ability to recognize CASP4 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Caspase 4 (CASP4) corresdonding to Asn78~Glu265 (Accession # P49662)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Caspase 4

Target: Caspase 4 (CASP4) (Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4))

Alternative Name: CASP4

Background: ICE(rel)II, ICH2, TX, Protease ICH-2, Cysteinyl Aspartate Specific Proteinases 4, Apoptosis-Related Cysteine Peptidase

UniProt: P49662

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity