ADAM12 antibody (N-Term)

Catalog No. ABIN374483

The Goat Polyclonal anti-ADAM12 antibody is suitable to detect ADAM12 in samples from Human. It has been validated for WB and IHC (p).

Quick Overview for ADAM12 antibody (N-Term) (ABIN374483)

Target: ADAM12 (ADAM Metallopeptidase Domain 12 (ADAM12))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ADAM12 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ADAM12 Antibody

Binding Specificity: N-Term

Sequence: AARPLPVSPA RALC

Specificity: This antibody is expected to recognize both the longer membrane-bound form of Human ADAM12 (NP_003465) and the shorter soluble ADAM12 splice isoform (NP_067673). It does not cross-react with other ADAMS.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from the N Terminus of the protein sequence according to NP_003465, NP_067673

Application Details

Application Notes: Peptide ELISA: Detection Limit Dilution: 1/64000. Western blot: 0.1-0.3 μg/mL. Approx 90 kDa band observed in human heart lysates

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ADAM12

Target: ADAM12 (ADAM Metallopeptidase Domain 12 (ADAM12))

Alternative Name: ADAM12 / MLTN

Background: ADAM12 is a member of the ADAM (a disintegrin and metalloprotease like domain) family. Two forms of ADAM12 have been described: ADAM12S and ADAM12L (short and long forms). The short form is a soluble form lacking the transmembrane and cytoplasmic domains. The short form of ADAM12 was reported to provoke myogenesis, and the lack of cytoplasmic domain suggests different regulation pathways (although both forms can be expressed in the same tissue). Other papers investigated the SH3 ligand domains in the cytoplasmic portion of ADAM12, demonstrating regulation routes for ADAM12 via Src and Src tyrosine kinase. ADAM12 is primarily expressed in muscle and bone (although placenta, osteoblasts, and many tumor cell lines also express ADAM12). ADAM12 supports cell adhesion by acting as a cell attachment molecule and binding integrins through the cysteine rich domain. A marker of skeletal muscle regeneration, it has been implicated in myoblast differentiation and fusion. ADAM12 contains the canonical HExxHxxxxxH zinc metalloproteinase motif. Full length ADAM12 (909 amino acids) has a predicted mass of 99.5 kDa, but due to glycosylation and cyteine rich regions, the reduced protein migrates to 114 kDa (unprocessed) and 84 kDa (processed). The soluble form of ADAM12 (738 amino acids, predicted mass of 80.5 kDa) is seen as a 92 kDa zymogen and 68 kDa furin processed form.Synonyms: Disintegrin and metalloproteinase domain-containing protein 12, Meltrin alpha

Gene ID: 8038, 9606

UniProt: O43184

Pathways: EGFR Signaling Pathway