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ADH1A/ADH1B/ADH1C (ADH1A/ADH1B/ADH1C) antibody

Antigen

ADH1A/ADH1B/ADH1C (ADH1A/ADH1B/ADH1C)

Clonality Polyclonal
Host

Goat

Reactivity

Chimpanzee

Application
Western Blotting (WB)
Catalog no. ABIN374506
Quantity 0.1 mg  (0.5 mg/ml)
Price 290.00 $   Plus shipping costs $35.00
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Additional Information

Characteristics Synonyms: Alcohol dehydrogenase subunit alpha, ADH1A
Alternative name ADH1A + ADH1B + ADH1C (pan ADH class I)
Gene ID 124
Swiss-Prot P07327
Immunogen Peptide with sequence STAGKVMKCKA, from the N Terminus of the protein sequenceaccording to NP_000658. 1, NP_000659. 2, NP_000660. 1.
Cross-Reactivity Chimpanzee, Horse (Equine), Human, Mouse (Murine), Rat (Rattus)
Description ADH (Alcohol dehydrogenase) consists of a dimer of identical or nonidentical chains ofthree types, alpha, beta and gamma. Members of this enzyme family metabolize a widevariety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids,and lipid peroxidation products. There are 7 different ADH's isozymes in human: three belongs to class I: alpha, beta, andgamma, one to class II: pi, one to class III: chi, one to class IV: ADH7 and one to class V: ADH6.
Specificity This antibody is expected to recognize the alpha (ADH1A, NP_ 000658. 1), the beta (ADH1B,NP_000659. 2) and gamma (ADH1C, NP_000660. 1) polypeptide variants of Human alcoholdehydrogenase. Species Reactivity: Tested: Human. Expected from sequence similarity: Mouse, Rat.

Application Details

Application Notes Peptide ELISA: 1/16000. Western Blot: 0.3-1.5 µg/ml. Approx 38kDa band observed in Human Liver lysates(calculated MW of 39.9kDa according to NP_000658.1, NP_000659.2 and 000660.1). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Concentration 0.5 mg/ml
Purification Aff - Purified
Buffer Tris saline, pH 7.3 containing 0.02% Sodium Azide as presrevative and0.5% BSA as stabilizer.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Restrictions For Research Use only