CCKAR antibody (Internal Region)

Catalog No. ABIN374568

The Goat Polyclonal anti-CCKAR antibody (ABIN374568) specifically detects CCKAR in IF, IHC (p) and EIA. The antibody is reactive with Human and Rat samples.

Quick Overview for CCKAR antibody (Internal Region) (ABIN374568)

Target: CCKAR (Cholecystokinin A Receptor (CCKAR))

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This CCKAR antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-CCKAR Antibody

Binding Specificity: Internal Region

Sequence: C-FEASQKKSAK ERKPST

Specificity: This antibody recognizes CCK-A Receptor.

Cross-Reactivity (Details): Species reactivity (tested):Human and Rat.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_000721.1.

Application Details

Application Notes: Peptide ELISA: 1/4000. Western Blot. Immunohistochemistry on paraffin sections: 10 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 with 0.02 % Sodium Azide and 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CCKAR

Target: CCKAR (Cholecystokinin A Receptor (CCKAR))

Alternative Name: CCK-A Receptor

Background: The cholecystokinin (CCK) family of peptide hormones have been implicated in numerous important physiologic events. These appear to be mediated through 2 general classes of receptors, A (CCKAR)and B (CCKBR), based on their binding affinities for CCK/gastrin family peptides. Through binding to class A receptors, CCK is a major physiologic mediator of gallbladder contraction and pancreatic enzyme secretion. It appears to play a role in slowing gastric emptying, relaxation of the sphincter of Oddi, and potentiation of insulin secretion. Further, it has been implicated as a mediator of pancreatic growth and tumorigenesis. Class A receptors have also been described in the anterior pituitary, myenteric plexus, and regions of the central nervous system, where they have been implicated in the pathogenesis of feeding disorders, Parkinson disease, schizophrenia, and drug addiction.Synonyms: CCKA Receptor, CCKAR, CCKRA, Cholecystokinin receptor type A, Cholecystokinin-1 receptor

Gene ID: 886, 9606

UniProt: P32238

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Feeding Behaviour