Glucose-6-Phosphate Dehydrogenase antibody (Internal Region)

Catalog No. ABIN374790

The Goat Polyclonal anti-Glucose-6-Phosphate Dehydrogenase antibody (ABIN374790) specifically detects Glucose-6-Phosphate Dehydrogenase in WB, IHC (p) and EIA. The antibody is reactive with Human, Mouse, Rat and Dog samples.

Quick Overview for Glucose-6-Phosphate Dehydrogenase antibody (Internal Region) (ABIN374790)

Target: Glucose-6-Phosphate Dehydrogenase (G6PD)

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This Glucose-6-Phosphate Dehydrogenase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-Glucose-6-Phosphate Dehydrogenase Antibody

Binding Specificity: AA 308-320, Internal Region

Sequence: C-STNSDDVRDE KVK

Specificity: This antibody is expected to recognise both reported isoforms (NP_000393.4 and NP_001035810.1).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat and Dog (Canine).
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography.

Immunogen: Synthetic Peptide from the internal region (aa 308-320) of Human G6PD. Genename: G6PD

Application Details

Application Notes: Peptide ELISA: Limit dilution: 1/32000. Western Blot: Use at 0.03-0.1 μg/mL. Detects a double band of approx. 55-60 kDa band in HumanTestis, Thyroid and Tonsil lysates. Immunohistochemistry: 2-4 μg/mL. In paraffin embedded Human Testis shows strongcytoplasm staining in spermatogonia.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month (in aliquots) at -20 °C for longer.

Target Details for Glucose-6-Phosphate Dehydrogenase

Target: Glucose-6-Phosphate Dehydrogenase (G6PD)

Alternative Name: G6PD

Background: Glucose 6 Phosphate Dehydrogenase (G6PD) produces pentose sugars for nucleic acid synthesis and is the main producer of NADPH reducing power. Catalytic activity: D glucose 6 phosphate + NADP(+) = D glucono 1,5 lactone 6 phosphate + NADPH. Defects in G6PD are the cause of chronic non spherocytic haemolytic anemia (CNSHA). G6PD deficiency is the most common human enzyme deficiency, one benefit of having G6PD deficiency is that it confers a resistance to malaria.Synonyms: Glucose-6-P-Dehydrogenase, Glucose-6-phosphate 1-dehydrogenase

Gene ID: 2539, 9606

UniProt: P11413

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones