CCKAR antibody (Internal Region)

Catalog No. ABIN374792

The Goat Polyclonal anti-CCKAR antibody is suitable to detect CCKAR in samples from Rat, Mouse and Rabbit. It has been validated for WB.

Quick Overview for CCKAR antibody (Internal Region) (ABIN374792)

Target: CCKAR (Cholecystokinin A Receptor (CCKAR))

Reactivity: Rat, Mouse, Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: This CCKAR antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CCKAR Antibody

Binding Specificity: Internal Region

Sequence: C-KFDASQKKSA KEKR

Specificity: This antibody detects CCK-A Receptor.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, RabbitSpecies reactivity (tested):Rat.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the internal region of the protein sequence according to NP_033957.1. Genename: Cckar

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1: 64000. Western blot: 0.1-0.3 μg/mL. Approx.75 kDa band observed in purified membranes of Ratinsulinoma cell line RIN14B (calculated MW of 48.5 kDa according to NP_033957.1). Datakindly provided by Dr. Morisset, Sherbrooke, Canada. Data accepted by InternationalJournal of Endocrinology (in press). The observed molecular weight also corresponds toearlier findings in literature with different antibodies (Morisset et al J Histochem Cytochem.2003 Nov,51(11): 1501-13, PMID: 14566022).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide, with 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CCKAR

Target: CCKAR (Cholecystokinin A Receptor (CCKAR))

Alternative Name: CCK-A Receptor

Background: The cholecystokinin (CCK) family of peptide hormones have been implicated in numerous important physiologic events. These appear to be mediated through 2 general classes of receptors, A (CCKAR)and B (CCKBR), based on their binding affinities for CCK/gastrin family peptides. Through binding to class A receptors, CCK is a major physiologic mediator of gallbladder contraction and pancreatic enzyme secretion. It appears to play a role in slowing gastric emptying, relaxation of the sphincter of Oddi, and potentiation of insulin secretion. Further, it has been implicated as a mediator of pancreatic growth and tumorigenesis. Class A receptors have also been described in the anterior pituitary, myenteric plexus, and regions of the central nervous system, where they have been implicated in the pathogenesis of feeding disorders, Parkinson disease, schizophrenia, and drug addiction.Synonyms: CCKA Receptor, CCKAR, CCKRA, Cholecystokinin receptor type A, Cholecystokinin-1 receptor

Gene ID: 886, 9606

UniProt: P32238

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Feeding Behaviour