PLIN1 antibody (C-Term)

Catalog No. ABIN374794

This Goat Polyclonal antibody specifically detects PLIN1 in WB, IHC (p), IF and IHC (fro). It exhibits reactivity toward Human, Rat and Dog.

Quick Overview for PLIN1 antibody (C-Term) (ABIN374794)

Target: PLIN1 (Perilipin 1 (PLIN1))

Reactivity: Human, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This PLIN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-PLIN1 Antibody

Binding Specificity: C-Term

Sequence: C-EPILGRAQYS QLRKK

Specificity: This antibody detects Perilipin-1 at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Rat, Dog.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Syntehtic peptide from the C-terminus of Human PLIN (NP_002657.2) Genename: PLIN

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1: 32000. Western blot: 0.01-0.03 μg/mL. Approx 55-60 kDa band observed in Human Adipose lysates(calculated MW of 56.0 kDa according to NP_002657.2). Immunofluorescence: 1 μg/mL. Small lipid droplets preferentially stained in the periphery ofdifferentiated 3T3-L1 adipocytes.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide and 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.

Target Details for PLIN1

Target: PLIN1 (Perilipin 1 (PLIN1))

Alternative Name: Perilipin-1

Background: Synonyms: Lipid Droplet-Associated Protein, PERI, PLIN, PLIN-1, PLIN1, Perilipin

Gene ID: 5346, 9606

UniProt: O60240

Pathways: Lipid Metabolism