PSME1 antibody (C-Term, Isoform 1)

Catalog No. ABIN374820

The Goat Polyclonal anti-PSME1 antibody (ABIN374820) specifically detects PSME1 in WB. The antibody is reactive with Human, Mouse, Rat and Dog samples.

Quick Overview for PSME1 antibody (C-Term, Isoform 1) (ABIN374820)

Target: PSME1 (Proteasome (Prosome, Macropain) Activator Subunit 1 (PA28 Alpha) (PSME1))

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This PSME1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PSME1 Antibody

Binding Specificity: C-Term, Isoform 1

Specificity: This antibody is expected to recognise isoform 1 (NP_006254.1).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat and Canine (Dog).
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KNFEKLKKPRGETK, from the C Terminus of the protein sequence

Application Details

Application Notes: Peptide ELISA: Detection Limit: 1/64000. Western Blot: 0.3-1 μg/mL. Approx 30 kDa band observed in Human Lymph Node, Spleen, Tonsil and Peripheral BloodMononuceocyte lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PSME1

Target: PSME1 (Proteasome (Prosome, Macropain) Activator Subunit 1 (PA28 Alpha) (PSME1))

Alternative Name: PSME1

Background: PSME1 is implicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.Synonyms: 11S regulator complex subunit alpha, Activator of multicatalytic protease subunit 1, IFI5111, IGUP I-5111, Interferon gamma up-regulated I-5111 protein, PA28a, PA28alpha, PSME-1, Proteasome activator 28 subunit alpha, Proteasome activator complex subunit 1, REG-alpha

Gene ID: 5720

NCBI Accession: NP_006254

UniProt: Q06323

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA