APOA4 antibody (C-Term, Internal Region)

Catalog No. ABIN374863

This Goat Polyclonal antibody specifically detects APOA4 in WB. It exhibits reactivity toward Human.

Quick Overview for APOA4 antibody (C-Term, Internal Region) (ABIN374863)

Target: APOA4 (Apolipoprotein A-IV (APOA4))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This APOA4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-APOA4 Antibody

Binding Specificity: C-Term, Internal Region

Sequence: C-KEKESQDKTL SLP

Specificity: This antibody detects Apolipoprotein A IV (ApoA IV).

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from the internal region (near the C-Terminus) of the protein sequence according to NP_000473.2 Genename: APOA4

Application Details

Application Notes: Peptide ELISA: Detection Limit Dilution: 1/32000. Western blot: 0.01-0.03 μg/mL. Approx 45 kDa band observed in Human Duodenum lysates(Calculated MW of 45.5 kDa according to NP_000473.2). Immunohistochemistry on Paraffin Sections: 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for APOA4

Target: APOA4 (Apolipoprotein A-IV (APOA4))

Alternative Name: Apolipoprotein A IV (ApoA IV)

Background: APOA4 (apolipoprotein A-IV) is a component of HDL and chylomicrons. Its primary site of synthesis is the intestine, in association with lymph chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase (LCAT) in vitro. In Rodents, Apo A-IV inhibits gastric emptying and serves as a satiety factor whose synthesis and secretion are increased by the ingestion of dietary fat. It also possesses anti-inflammatory and antiatherogenic properties.Synonyms: APOA4, Apo-AIV, ApoA-IV, Apolipoprotein A-IV

Gene ID: 9606

UniProt: P06727

Pathways: Lipid Metabolism