G-Protein Signaling Modulator 2 (GPSM2) antibody

Antigen: G-Protein Signaling Modulator 2 (GPSM2)

Synonyms: LGN, Pins, 6230410J09Rik, gpsm2, MGC52811

Clonality: Polyclonal

Host: Goat

Reactivity: Dog (Canine)

Application: Western Blotting (WB)

Catalog no.: ABIN374868

Additional Information

Characteristics: Synonyms: GPSM-2, LGN, G-protein-signaling modulator 2, Mosaic protein LGN

Alternative name: GPSM2

Swiss-Prot: P81274

Immunogen: Peptide from the internal region of the protein sequence according to NP_037428. 2Genename: GPSM2AA Sequence: C-NRLKGKKYKTNSSTK

Cross-Reactivity: Dog (Canine), Human

Description: GPSM2 plays an important role in spindle pole orientation. It interacts and contributes tothe functional activity of G(i) alpha proteins. GPSM2 acts to stabilize the apical complexduring neuroblast divisions.

Specificity: This antibody detects GPSM2. Species Reactivity: Tested: HumanExpected from sequence similarity: Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1: 64000. Western blot: 0.01-0.03µg/ml. Approx 70kDa band observed in Human Brain (Cerebellum)and Human Liver lysates (calculated MW of 75.8kDa according to NP_037428.2). Anadditional band of unknown identity was also consistently observed at 26-28kDa. Thisband was successfully blocked by incubation with the immunising peptide. We wouldappreciate any feedback from people in the field - have any such results been reportedwith other antibodies/lysates? Have any further splice variants/modified forms beenreported?Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Aff - Purified

Buffer: Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin

Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Signaling

Restrictions: For Research Use only