FZD7 antibody (C-Term)

Catalog No. ABIN374921

The Goat Polyclonal anti-FZD7 antibody is suitable to detect FZD7 in samples from Human and Mouse. It has been validated for WB.

Quick Overview for FZD7 antibody (C-Term) (ABIN374921)

Target: FZD7 (Frizzled Family Receptor 7 (FZD7))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This FZD7 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-FZD7 Antibody

Binding Specificity: C-Term

Sequence: C-RFYHRLSHSS KGET

Specificity: This antibody recognizes Frizzled 7 (C-term).

Cross-Reactivity (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RFYHRLSHSSKGET from the C Terminus of Human FZD7 (NP_003498.1).

Application Details

Application Notes: Peptide ELISA: Detection Limit Dilution: 1/64000. Western Blot: 0.3-1 μg/mL. Approx 60 kDa band observed in lysates of cell lines HEK293 andHepG2 (calculated MW of 63.0 kDa according to NP_003498.1). Immunohistochemistry on Paraffin Sections: 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for FZD7

Target: FZD7 (Frizzled Family Receptor 7 (FZD7))

Alternative Name: FZD7 / Frizzled-7

Background: Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The Frizzled 7 protein contains an N-terminal signal sequence, 10 cysteine residues typical of the cysteine-rich extracellular domain of Frizzled family members, 7 putative transmembrane domains, and an intracellular C-terminal tail with a PDZ domain-binding motif. Frizzled 7 gene expression may downregulate APC function and enhance beta-catenin-mediated signals in poorly differentiated human esophageal carcinomas. Frizzled 7 expression has been reported in brain, gastrointestinal tract, heart, fetal kidney, fetal lung, placenta, skeletal muscle, and various cancers. ESTs have been isolated from a wide variety of normal and cancer libraries.Synonyms: Fz-7, mFz7

Gene ID: 8324

NCBI Accession: NP_003498

UniProt: O75084

Pathways: WNT Signaling, Stem Cell Maintenance