Clusterin antibody (C-Term)

Catalog No. ABIN375042

Cited in 1 publication. The Goat Polyclonal anti-Clusterin antibody (ABIN375042) specifically detects Clusterin in WB and EIA. The antibody is reactive with Rat and Mouse samples.

Quick Overview for Clusterin antibody (C-Term) (ABIN375042)

Target: Clusterin (CLU)

Reactivity: Rat, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This Clusterin antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Clusterin Antibody

Binding Specificity: C-Term

Sequence: C-EKALQEYRRK SRAE

Specificity: This antibody recognizes Clusterin / ApoJ (C-term).

Cross-Reactivity (Details): Species reactivity (tested):Mouse, Rat.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the C Terminus of the protein sequence according to NP_038520.1. Genename: Clu

Application Details

Application Notes: Peptide ELISA: 1/8000. Western Blot: 0.3-1 μg/mL. Approx.37 kDa band observed in Mouse Brain and Testis lysatesand in Rat Brain and Ovary lysates (calculated MW of 51.7 kDa according to Mouse

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris buffered saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-Clusterin antibody (ABIN375042)

Charnay, Imhof, Vallet, Hakkoum, Lathuiliere, Poku, Aronow, Kovari, Bouras, Giannakopoulos: "Clusterin expression during fetal and postnatal CNS development in mouse." in: Neuroscience, Vol. 155, Issue 3, pp. 714-24, (2008) (PubMed).

Target Details for Clusterin

Target: Clusterin (CLU)

Alternative Name: Apolipoprotein J (Apo J)

Background: At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individual's relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL). Apolipoprotein J is the major secreted product of Sertoli cells and is thought to play a critical role in spermatogenesis. The protein was shown to be a normal constituent of human blood and is also thought to be a control mechanism of the complement cascade. It prevents the binding of a C5b-C7 complex to the membrane of the target cell and in this way inhibits complement-mediated cytolysis. Apolipoprotein J is overexpressed in human prostate and breast cancers and in squamous cell carcinomas. Suppression of Apolipoprotein J renders these cells sensitive to chemotherapeutic drug-mediated apoptosis. It has been proposed that elevated levels of Apolipoprotein J may promote oncogenic transformation and tumor progression by interfering with BAX proapoptotic activities.Synonyms: 40, 40, AAG4, APOJ, Aging-associated gene 4 protein, CLI, Clusterin, Complement cytolysis inhibitor, Complement-associated protein SP-40, KUB1, Ku70-binding protein 1, NA1/NA2, SP-40, Testosterone-repressed prostate message 2

Gene ID: 12759, 10090

UniProt: Q06890

Pathways: Apoptosis, Negative Regulation of intrinsic apoptotic Signaling