Biglycan antibody (C-Term)

Catalog No. ABIN375174

The Goat Polyclonal anti-Biglycan antibody (ABIN375174) specifically detects Biglycan in WB and EIA. The antibody is reactive with Human, Rat, Mouse and Dog samples.

Quick Overview for Biglycan antibody (C-Term) (ABIN375174)

Target: Biglycan (BGN)

Reactivity: Human, Rat, Mouse, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This Biglycan antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Biglycan Antibody

Binding Specificity: C-Term

Sequence: C-DRLAIQFGNY KK

Specificity: This antibody recognizes Biglycan Preproprotein.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Canine.
Species reactivity (tested):Human, Rat.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Synthetic peptide from the C-Terminus of Human Biglycan (NP_001702.1) Genename: BGN

Application Details

Application Notes: Peptide ELISA: 1/8000. Western Blot: 0.1 - 0.3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Biglycan

Target: Biglycan (BGN)

Alternative Name: Biglycan

Background: Biglycan is an extracellular matrix proteoglycan that is closely related in structure to decorin and fibromodulin. Biglycan and decorin are thought to be the result of a gene duplication. Biglycan is expressed in secondary myotubes during fetal muscle formation. In adult muscle it is located in the endomysium and neuromuscular junctions, where it connects to the dystrophin-associated proteins through binding to a-dystroglycan. In developing newborn mice biglycan expression is initially high and then decreases during skeletal muscle differentiation and maturation. Biglycan may also have a role in the fibrotic process since it is overexpressed in atherosclerotic lesions and has been shown to cause an increase in thymidine incorporation and migration of vascular smooth muscle cells.Synonyms: BGN, Bone/cartilage proteoglycan I, PG-S1, PGS1, SLRR1A, Small Leucine-rich Protein 1A

Molecular Weight: 41.7 kDa (Human NP_001702.1 and Rat NP_058783.1)

Gene ID: 633

NCBI Accession: NP_001702

UniProt: P21810

Pathways: Glycosaminoglycan Metabolic Process