BNIP1 antibody (Internal Region, Isoform 1)

Catalog No. ABIN375177

The Goat Polyclonal anti-BNIP1 antibody (ABIN375177) specifically detects BNIP1 in WB and EIA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for BNIP1 antibody (Internal Region, Isoform 1) (ABIN375177)

Target: BNIP1 (BCL2/adenovirus E1B 19kDa Interacting Protein 1 (BNIP1))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This BNIP1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-BNIP1 Antibody

Binding Specificity: Internal Region, Isoform 1

Specificity: This antibody reacts to BNIP1. All reported variants represent isoform 1.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Peptide with sequence C-DLLRQRKTTKESLAQ, from the internal region of the protein sequence

Application Details

Application Notes: Peptide ELISA: 1/8000. Western Blot: 0.5 - 1.5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0,5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for BNIP1

Target: BNIP1 (BCL2/adenovirus E1B 19kDa Interacting Protein 1 (BNIP1))

Alternative Name: BNIP1

Background: BNIPs belong to the proapoptotic BH3-only protein family. Three isoforms have been described so far. BNIP 1 is involved in the maintenance of ER-integrity. It is a member of the syntaxin 18 complex which is involved in membrane trafficking between the ER and Golgi. Four different BNIP 1 splice variants have been described so far, one of which contains a highly conserved BH3 domain. All BNIP 1 variants are able to interact with the apoptosis regulators BCL2 and BCL2L1.Synonyms: BCL2/adenovirus E1B 19 kDa protein-interacting protein 1, NIP1, SEC20L, TRG8, Transformation-related gene 8 protein, Vesicle transport protein SEC20

Gene ID: 662

NCBI Accession: NP_001196

UniProt: Q12981