CD8 alpha antibody

Catalog No. ABIN375493

This Rat Monoclonal antibody specifically detects CD8 alpha in FACS, IP, ICC, IHC (fro), BR and Dep. It exhibits reactivity toward Mouse.

Quick Overview for CD8 alpha antibody (ABIN375493)

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD8 alpha antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Blocking Reagent (BR), Depletion (Dep)

Clone: 53-6-7

Product Details anti-CD8 alpha Antibody

Purpose: Rat Anti-Mouse CD8α-LE/AF

Specificity: Mouse CD8α, Mr 32-34 kDa

Characteristics: Low Endotoxin/Azide Free Anti-Mouse CD8 alpha antibody for use in flow cytometry, immunohistochemistry / immunocytochemistry, immunoprecipitation, depletion, and blocking assays.

Endotoxin Level: Low Endotoxin

Immunogen: Spleen cells or thymocyte membranes

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Phosphate buffered saline, pH 7.4

Preservative: Azide free

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: 2-8°C

Target Details for CD8 alpha

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Alternative Name: CD8alpha

Background: In the mouse, CD8 exists in two forms: (i) a CD8 heterodimer composed of an α chain (CD8 α /Lyt-2) and a β chain (CD8 β /Lyt-3), and (ii) a homodimer of two α chains. The heterodimer is found on the surface of essentially all thymocytes and the 'suppressor/cytotoxic' subpopulation of mature T lymphocytes. Subsets of intestinal intraepithelial lymphocytes express CD8 α without CD8 β. It has been suggested that CD8 + β - T cells mature extrathymically, while development of the CD8 α + β + population of T cells is thymus-dependent. CD8 acts as a coreceptor with MHC Class I-restricted T cell receptors in antigen recognition and positive selection of MHC class I-restricted CD8 + T cells

Gene ID: 12525

UniProt: P01731

Pathways: TCR Signaling