Phosphotyrosine (0) antibody (FITC)

Antigen: Phosphotyrosine (0)

Clonality: Monoclonal (PY20)

Host: Mouse

Conjugate: FITC

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Catalog no.: ABIN376118

Additional Information

Alternative name: Phosphotyrosine

Isotype: IgG2b

Clone: PY20

Specificity: Phosphorylated tyrosine and phenylphosphate Protein tyrosine residues are phosphorylated as a result of intracellular protein kinase activation (e.g. via growth factors) during normal growth and development, and in oncogenesis. The most abundant population of target proteins for tyrosine phosphorylation are cell surface glycoproteins. Antibody to phosphotyrosine enables the detection, isolation and characterization of proteins containing phosphotyrosine. PY20 also prevents internalization of activated receptors, e.g. EGFr, when microinjected into cells. The affinity of PY20 for phosphotyrosine is approximately 10 -6 to 10 -7 M. PY20 binding to phosphorylated tyrosines can be inhibited by free phosphotyrosine and phenylphosphate, but not by phosphoserine, phosphothreonine or free phosphate.

Application Details

Application Notes: To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested to conform to characteristics of a standard reference reagent

Buffer: The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8°C. The fluorescein (FITC) conjugate is supplied as 0.5

Storage: Store at 2-8°C

Research Area: Signaling, Metabolism, Amino Acids, Protein Modifications

Restrictions: For Research Use only