CD3 (CD3E) antibody

Antigen: CD3 (CD3E)

Synonyms: T3E, TCRE, FLJ18683

Clonality: Monoclonal (UCHT1)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS)

Catalog no.: ABIN376613

Additional Information

Alternative name: CD3

Isotype: IgG1  (Matching secondary antibodies)

Clone: UCHT1

Specificity: Epsilon chain of the CD3/T cell antigen receptor complex CD3, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains ranging from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). CD3 is expressed on 70-80% of normal peripheral blood lymphocytes and on 10-20% of thymocytes. It plays a major role in signaling during antigen recognition, leading to T cell activation. The monoclonal antibody UCHT1 reacts with the 20 kDa epsilon chain of the CD3/TCR complex.

Application Details

Application Notes: To insure lot to lot consistency, each batch of product is tested by flow cytometry to conform to the characteristics of a standard reference reagent. Purified antibody conjugate: <= 1 µ g/10 6 cells, Fluorescein conjugate: 10 µ L/10 6 cells, Biotin conjugate: 10 µ L/10 6 cells, R-phycoerythrin conjugate: 10 µ L/10 6 cells, Allophycocyanin and Allophycocyanin-Cyanine 7 conjugate: 10 µ L/10 6 cells, Spectral Red conjugate: 10 µ L/10 6 cells, Cyanine 5 conjugate: 10 µ L/10 6 cells, R-phycoerythrin-Cyanine 5.5 conjugate: 10 µ L/10 6 cells, R-phycoerythrin-Cyanine 7 conjugate: 10 µ L/10 6 cells, AF488 and AF647 conjugate: 10 µ L/10 6 cells.

Buffer: The purified (UNLB) antibody is supplied as 0.1 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added. Store at 2-8°C. The fluorescein (FITC), Cyanine 5 (CY 5), Alexa Flu

Storage: Store at 2-8°C

Research Area: Immunology, Adaptive Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only