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CD3 (CD3E) antibody (SPRD)

Antigen

CD3 (CD3E)
Synonyms T3E, TCRE, FLJ18683
Clonality Monoclonal (CT-3)
Host
Alternatives

Mouse
Reactivity
Alternatives

Chicken
Conjugate
Application
Alternatives Flow Cytometry (FACS)
2 references available
Catalog no. ABIN376729
Quantity 0.1 mg
Price 290.00 $   Plus shipping costs $35.00
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Additional Information
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Alternative name CD3
Isotype IgG1
Clone CT-3
Specificity Chicken CD3 CD3 is a member of the T cell receptor-associated CD3 complex. MAb CT-3 recognizes a complex of at least three polypeptides of Mr 20-, 19-, and 17-kDa (two of which are N-glycosylated) on chicken T cells. The antibody also coprecipitates a polypeptide of 90-kDa from digitonin solubilized T cell lysates, which can be reduced to two polypeptides of Mr 50- and 40-kDa.

Application Details
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Application Notes To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of a standard reference. Representative data are included in this product insert. Fluorescein conjugate: <= 1 µ g/10 6 cells, Biotin conjugate: <= 1 µ g/10 6 cells, R-phycoerythrin conjugate: <= 0.5 µ g/10 6 cells, Spectral Red conjugate: <= 0.2 µ g/10 6 cells.
Buffer The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8°C. The fluorescein (FITC) conjugate is supplied as 0.5
Storage Store at 2-8°C
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Surface Receptors of Immune Cells
Restrictions For Research Use only

Publications
Application Details Alternatives back to top

Publications Dalgaard, Norup, Pedersen et al.: "Flow cytometric assessment of chicken T cell-mediated immune responses after Newcastle disease virus vaccination and challenge." in: Vaccine, 2010 (PubMed).

Carballeda, Zoth, Gómez et al.: "Activation of the immune response against Infectious Bursal Disease Virus after intramuscular inoculation of an intermediate strain." in: Immunobiology, Vol. 216, Issue 9, pp. 1028-33, 2011 (PubMed).

Alternatives
Application Details Publications back to top

Alternatives for antigen "CD3 (CD3E)", type "Antibodies"
Hosts Mouse (309), Rat (55), Rabbit (51), Hamster (38)
Reactivities Human (293), Mouse (Murine) (105), Rat (Rattus) (43), Dog (Canine) (30), Pig (Porcine) (26), Chicken (17), Cow (Bovine) (17), Monkey (15), Primate (9), Bird (Avian) (4), Cat (Feline) (4), Hamster (3), Horse (Equine) (3), Primates (3), Rhesus Monkey (2), Rabbit (1)
Applications Flow Cytometry (FACS) (353), Immunohistochemistry (Frozen Sections) (IHC (fro)) (92), Immunoprecipitation (IP) (66), Functional Studies (Func) (49), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (49), Western Blotting (WB) (42), Immunofluorescence (IF) (32), ELISA (19), Immunohistochemistry (IHC) (17), Blocking Antibody (Inhibition) (10), Enzyme Immunoassay (EIA) (7), Immunocytochemistry (ICC) (6), Cytotoxicity Test (CyTox) (2), Dot Blot (Dot) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Radioimmunoassay (RIA) (2), Immunoelectron Microscopy (IEM) (1)
Conjugates FITC (76), Biotin (46), PE (39), APC (24), RPE (10), Cy5 (6), SPRD (6), Alexa Fluor 488 (4), Cy5,PE (4), DY647 (3), PE,Cy5 (3), PE,Cy7 (3), APC,Cy7 (2), Alexa Fluor 647 (2), Cy7,PE (2), PE,Cy5.5 (2), PE,DY647 (2), PE-Dyomics 647 (2), PerCP (2), RPE,Cy5 (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 555 (1), Alexa Fluor 700 (1), Cy3 (1), Cy5.5 (1), Cy7 (1), Gold (1), HRP (1), PE,Cy3 (1), Pacific Blue (1), RBITC (1), RPE,Cy7 (1)
Epitopes AA 156-168 (4), AA 196-206 (1), C-Term (1)