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Actin, beta (ACTB) antibody (HRP)
| Antigen | Actin, beta (ACTB) |
| Synonyms |
PS1TP5BP1, Actx, beta-actin, E430023M04Rik, ACTB, MGC128179, MGC76228, MGC79692, MGC88999, ps1tp5bp1, LOC442937, LOC563571, A26C1A, A26C1B, ACT-5, act-2, Actb, actb, B-actin, LOC100136013, DKFZp459A19 ...
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| Clonality | Monoclonal (2D1D10) |
| Host |
Alternatives Mouse |
| Conjugate |
Alternatives HRP |
| Application |
Alternatives ELISA, Western Blotting (WB)
|
| Certificates | ISO 9001:2008 |
| Catalog no. | ABIN387705 |
| Quantity | 200ug (5 x 40ug) (40 ug, lyophilized with PBS, pH 7.4...) (Variants) |
| Price | 333.00 $ Plus shipping costs $35.00 |
| Shipping to |
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| Availability | Ships within 5 to 7 Business Days |
Additional Information
| Alternative name | beta actin |
| Immunogen | A synthetic peptide DDDIAALVVDNGSG coupled to KLH |
| Format | Purified |
| Isotype | IgG2a |
| Clone | 2D1D10 |
| Description | Antibodies-Online Anti-beta-Actin [HRP] Monoclonal Antibody (Mouse) is HRP conjugated Anti-beta-Actin Monoclonal Antibody (Mouse) (ABIN396859) purified from mice ascites by protein G affinity column. This product provides a useful and serves as a specific tool in the study of the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton. Do not add azide because it inhibits the activity of HRP. |
| Specificity | Anti-beta-Actin [HRP] Monoclonal Antibody (Mouse) reacts with mouse, rabbit, chicken, human, hamster, cow, goat, fish, and pig substrates. It has not yet been tested in other species. |
| Synonyms | PS1TP5BP1, Actx, beta-actin, E430023M04Rik, ACTB, MGC128179, MGC76228, MGC79692, MGC88999, ps1tp5bp1, LOC442937, LOC563571, A26C1A, A26C1B, ACT-5, act-2, Actb, actb, B-actin, LOC100136013, DKFZp459A196, LOC100174883, LOC100304816, ACTA1 |
Application Details
| Application Notes | Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.ELISA: 0.1-1.0 ug/ml Western blot: 0.1-1.0 ug/ml Other applications: user-optimized |
| Concentration | 40 ug, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal |
| Purification | Protein G affinity column |
| Storage | Anti-beta-Actin [HRP] Monoclonal Antibody (Mouse) should be stored lyophilized until use. It is stable in lyophilized form for at least two years if stored at -20°C. The reconstituted antibody can be stored for up to three months at 2-8°C or for up to two years at -20°C or below. Avoid repeated freeze and thaw cycles. |
| Restrictions | For Research Use only |
Images
| Detection antibody:1 µg/ml Anti-beta-Actin [HRP] Monoclonal Antibody (Mouse) (Antibodies-Online, ABIN396861)The signal was developed with LumiSensor HRP Substrate Kit (Antibodies-Online, L00221V500). |
Alternatives
Alternatives for antigen "Actin, beta (ACTB)", type "Antibodies"
| Hosts | Rabbit (35), Mouse (31), Chicken (2) |
| Reactivities | Human (57), Rat (Rattus) (26), Mouse (Murine) (24), Chicken (11), Rabbit (8), Cow (Bovine) (7), Pig (Porcine) (6), Sheep (Ovine) (5), Cat (Feline) (3), Dog (Canine) (3), Guinea Pig (3), Hamster (3), Monkey (3), Carp (2), Fish (2), Fruit Fly (Drosophila melanogaster) (2), Goat (2), Primate (1) |
| Applications | Western Blotting (WB) (66), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (20), Immunofluorescence (IF) (17), ELISA (14), Enzyme Immunoassay (EIA) (14), Immunohistochemistry (IHC) (6), Immunohistochemistry (Frozen Sections) (IHC (fro)) (5), Immunoprecipitation (IP) (5), Dot Blot (Dot) (4), Immunocytochemistry (ICC) (4), Flow Cytometry (FACS) (2), ELISA (Detection) (1) |
| Conjugates | Biotin (1), HRP (1) |
| Epitopes | N-Term (7), C-Term (1) |




Alternatives