Chloride Intracellular Channel 4 (CLIC4) antibody

Antigen: Chloride Intracellular Channel 4 (CLIC4)

Synonyms: H1, huH1, p64H1, CLIC4L, MTCLIC, FLJ38640, DKFZp566G223, cb142, wu:fb33c05, wu:fc30h10, clic4, MGC89006, MGC79614, CLIC4, DKFZp459A0325

Clonality: Monoclonal (17CT9-2-8)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN387769

Additional Information

Alternative name: CLIC4

Gene ID: 25932

UniProt: NP_039234

Immunogen: Purified His-tagged CLIC4 protein was used to produced this monoclonal antibody.

Isotype: IgG1 kappa

Clone: 17CT9-2-8

Description: Other names: Chloride intracellular channel protein 4, Intracellular chloride ion channel protein p64H1, CLIC4

Characteristics: Unpurified Mouse Monoclonal Antibody (Mab)

Specificity: Purified His-tagged CLIC4 protein was used to produced this monoclonal antibody.

Molecular Weight: 29 kDA

Comments:

Background: Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 4 (CLIC4) protein, encoded by the CLIC4 gene, is a member of the p64 family, the gene is expressed in many tissues and exhibits a intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells).

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:2000 Immunohistochemistry ~~ 1:50~100

Concentration: Ascites

Buffer: Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells, Cell Signaling, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-CLIC4 Monoclonal Antibody (Cat.#ABIN387769) in Hela cell line lysates. CLIC4(arrow) was detected using the ascites Mab. Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with CLIC4 Antibody (Cat.#ABIN387769), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry, clinical relevance has not been evaluated.

anti-Chloride Intracellular Channel 4 (CLIC4) antibody (Image 2)

Publications

Product: Yao, Qu, Yang et al.: "CLIC4 mediates TGF-beta1-induced fibroblast-to-myofibroblast transdifferentiation in ovarian cancer." in: Oncology reports, Vol. 22, Issue 3, pp. 541-8, 2009 (PubMed).

Spiekerkoetter, Guignabert, de Jesus Perez et al.: "S100A4 and bone morphogenetic protein-2 codependently induce vascular smooth muscle cell migration via phospho-extracellular signal-regulated kinase and chloride intracellular channel 4." in: Circulation research, Vol. 105, Issue 7, pp. 639-47, 13 p following 647, 2009 (PubMed).

Ponsioen, van Zeijl, Langeslag et al.: "Spatiotemporal regulation of chloride intracellular channel protein CLIC4 by RhoA." in: Molecular biology of the cell, Vol. 20, Issue 22, pp. 4664-72, 2009 (PubMed).

Li, Li, Jiang et al.: "Mutation detection in candidate genes for benign familial infantile seizures on a novel locus." in: The International journal of neuroscience, Vol. 120, Issue 3, pp. 217-21, 2010 (PubMed).

Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).