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Histone H3 antibody

Antigen

Histone H3
Synonyms
H3t, H3.4, H3/g, H3FT, MGC126886, MGC126888, Hist1, H3.2-221, DmelCG31613, CG31613, h3.2, h3/m, h3f2, h3fm, hist1h3g, H3F3, H3.3A, H3F3B, MGC87782, MGC87783, H3.3B, H3F3A, h3f3a, MGC52829, MGC86731, M ... show more
Clonality Monoclonal (44AT53-87)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human
Application
Alternatives Western Blotting (WB), ELISA
Certificates ISO 9001:2008
Catalog no. ABIN387776
Quantity 0.1 mg  (0.25 mg/ml)
Price 383.50 $   Plus shipping costs $35.00
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anti-Histone H3 antibody

Additional Information
Application Details Images Alternatives Secondary Antibodies back to top

Characteristics Purified Mouse Monoclonal Antibody (Mab)
Alternative name Phospho-Histone H3-S10
Gene ID 3021
Swiss-Prot P84243
Immunogen This monoclonal antibody is generated from mice immunized with a KLH conjugated synthetic peptide corresponding to sequences in AA7-18 of human histone H3.
Isotype IgG1  (Matching secondary antibodies)
Clone 44AT53-87
Description Other names: Histone H3
Specificity This monoclonal antibody is generated from mice immunized with a KLH conjugated synthetic peptide corresponding to sequences in AA7-18 of human histone H3.
Molecular Weight 17000 DA
Synonyms H3t, H3.4, H3/g, H3FT, MGC126886, MGC126888, Hist1, H3.2-221, DmelCG31613, CG31613, h3.2, h3/m, h3f2, h3fm, hist1h3g, H3F3, H3.3A, H3F3B, MGC87782, MGC87783, H3.3B, H3F3A, h3f3a, MGC52829, MGC86731, MGC165454, zgc:56193, zgc:86731, wu:fb58e10, FLJ35099, FLJ56780, FLJ56787, MGC131639, MGC89877, HIST1H3A

Application Details
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Application Notes ELISA~~1:1,000 Western blotting~~1:100~500
Concentration 0.25 mg/ml
Purification Purified
Buffer Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cancer, Signaling, Chromatin, Cell Structure
Restrictions For Research Use only

Alternatives
Application Details Images Secondary Antibodies back to top

Alternatives for antigen "Histone H3", type "Antibodies"
Hosts Rabbit (137), Mouse (14), Sheep (4)
Reactivities Human (144), Rat (Rattus) (74), Mouse (Murine) (73), Cow (Bovine) (23), Pig (Porcine) (19), Rabbit (19), Fruit Fly (Drosophila melanogaster) (17), Dog (Canine) (7), Chicken (4), Horse (Equine) (3), Sheep (Ovine) (3), Guinea Pig (1), Tetrahymena (1)
Applications Western Blotting (WB) (95), Immunofluorescence (IF) (75), Flow Cytometry (FACS) (49), ELISA (42), Enzyme Immunoassay (EIA) (29), Dot Blot (Dot) (23), Immunohistochemistry (IHC) (21), Chromatin Immunoprecipitation (ChiP) (19), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (16), Immunoprecipitation (IP) (11), Immunocytochemistry (ICC) (7), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
Conjugates Alexa Fluor 488 (8), Alexa Fluor 350 (7), Alexa Fluor 555 (7), Alexa Fluor 647 (7), PE,Cy5.5 (7), Agarose Beads (1), Alexa Flour 488 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1), RBITC (1)
Epitopes pSer10 (23), pSer28 (17), pThr3 (12), pThr11 (6), 3meLys79 (2), Mono Methyl Lysine 4 (2), N-Term (2), Tri Methyl Lysine 4 (2), 2meArg17 (1), 2meLys27 (1), 2meLys27, 3meLys27 (1), 2meLys36 (1), 2meLys4 (1), 2meLys79 (1), 3meLys27 (1), 3meLys27, pSer10 (1), 3meLys4 (1), 3meLys9 (1), 3meLys9, pSer10 (1), Ab-23 (1), Ab-3 (1), Acetyl-Lys14 (1), Acetyl-Lys18 (1), Acetyl-Lys23 (1), Acetyl-Lys27 (1), Acetylated (1), Arg2 (1), acLys9 (1), acLys9, pSer10 (1), acetyl-Lys9 (1), meLys27 (1), meLys36 (1), meLys4 (1), meLys79 (1), meLys9 (1)