UBE2I antibody

Catalog No. ABIN387788

There are 3+ publications for this product available. The Mouse Monoclonal anti-UBE2I antibody is suitable to detect UBE2I in samples from Human, Mouse and Rat. It has been validated for WB.

Quick Overview for UBE2I antibody (ABIN387788)

Target: UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This UBE2I antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 67AT1273-95-90

Product Details anti-UBE2I Antibody

Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.

Immunogen: This UBC9 antibody was raised using purified GST-UBC9 fusion protein.

Isotype: IgG1

Application Details

Application Notes: WB: 1:1000-1:2000. WB: 1:100~500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-UBE2I antibody (ABIN387788)

Maarifi, Hannoun, Geoffroy, El Asmi, Zarrouk, Nisole, Blondel, Chelbi-Alix: "MxA Mediates SUMO-Induced Resistance to Vesicular Stomatitis Virus." in: Journal of virology, Vol. 90, Issue 14, pp. 6598-6610, (2017) (PubMed).

Dutrieux, Portilho, Arhel, Hazan, Nisole: "TRIM5? is a SUMO substrate." in: Retrovirology, Vol. 12, pp. 28, (2015) (PubMed).

Wu, Jeng, Lai: "The SUMOylation of matrix protein M1 modulates the assembly and morphogenesis of influenza A virus." in: Journal of virology, Vol. 85, Issue 13, pp. 6618-28, (2011) (PubMed).

Target Details for UBE2I

Target: UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))

Alternative Name: UBC9

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene.

Molecular Weight: 18007

Gene ID: 7329

NCBI Accession: NP_003336, NP_919235, NP_919236, NP_919237

UniProt: P63279

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Ubiquitin Proteasome Pathway