Lin-28 Homolog A (C. Elegans) (LIN28A) antibody

Antigen: Lin-28 Homolog A (C. Elegans) (LIN28A)

Synonyms: CSDD1, LIN28, LIN-28, ZCCHC1, FLJ12457, Lin28, Tex17, Lin-28, Gm10299, AL024421, ENSMUSG00000070700, csdd1, lin-28, lin28a, zcchc1, lin28, MGC55584, sb:cb650, zgc:55584, RGD1566408, MGC97527

Clonality: Monoclonal (55CT58-12-1)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN387790

Additional Information

Alternative name: LIN28A

Gene ID: 79727

UniProt: Q9H9Z2

Immunogen: LIN28A recombinant protein is used to produce this monoclonal antibody.

Isotype: IgG1 kappa

Clone: 55CT58-12-1

Description: Other names: Lin-28 homolog A, Zinc finger CCHC domain-containing protein 1, LIN28, CSDD1, LIN28A, ZCCHC1

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: LIN28A recombinant protein is used to produce this monoclonal antibody.

Molecular Weight: 22743 DA

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:10000 Western blotting~~ 1:200~2000 Immunohistochemistry ~~ 1:50~200

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Translation Factors, Stem Cells, Signaling

Restrictions: For Research Use only

Images

Western blot analysis of LIN28 recombinant protein by anti-LIN28A Monoclonal Antibody (Cat.#ABIN387790). LIN28 (arrow) was detected using the purified Mab. Formalin-fixed and paraffin-embedded human testis tissue reacted with LIN28A Monoclonal Antibody (Cat.#ABIN387790), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry, clinical relevance has not been evaluated.

anti-Lin-28 Homolog A (C. Elegans) (LIN28A) antibody (Image 2)

Publications

Product: West, Viswanathan, Yabuuchi et al.: "A role for Lin28 in primordial germ-cell development and germ-cell malignancy." in: Nature, Vol. 460, Issue 7257, pp. 909-13, 2009 (PubMed).

Heo, Joo, Kim et al.: "TUT4 in concert with Lin28 suppresses microRNA biogenesis through pre-microRNA uridylation." in: Cell, Vol. 138, Issue 4, pp. 696-708, 2009 (PubMed).

Iliopoulos, Hirsch, Struhl: "An epigenetic switch involving NF-kappaB, Lin28, Let-7 MicroRNA, and IL6 links inflammation to cell transformation." in: Cell, Vol. 139, Issue 4, pp. 693-706, 2009 (PubMed).

Qiu, Ma, Wang et al.: "Lin28-mediated post-transcriptional regulation of Oct4 expression in human embryonic stem cells." in: Nucleic acids research, Vol. 38, Issue 4, pp. 1240-8, 2010 (PubMed).

Peng, Maihle, Huang: "Pluripotency factors Lin28 and Oct4 identify a sub-population of stem cell-like cells in ovarian cancer." in: Oncogene, Vol. 29, Issue 14, pp. 2153-9, 2010 (PubMed).