MAP1LC3A antibody

Catalog No. ABIN387792

This anti-MAP1LC3A antibody is a Mouse Monoclonal antibody detecting MAP1LC3A in WB. Suitable for Human, Mouse and Rat. This Primary Antibody has been cited in 17+ publications.

Quick Overview for MAP1LC3A antibody (ABIN387792)

Target: MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This MAP1LC3A antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 166AT1234

Product Details anti-MAP1LC3A Antibody

Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.

Immunogen: This LC3 antibody is generated from mouse immunized with a full length recombinant protein of human LC3 (APG8).

Isotype: IgG1 kappa

Application Details

Application Notes: WB: 1:8000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Expiry Date: 6 months

References for anti-MAP1LC3A antibody (ABIN387792)

Ichikawa, Alves, Pfeiffer, Langa, Hernández-Santana, Suzuki, Prehn, Engel, Henshall: "Deletion of the BH3-only protein Noxa alters electrographic seizures but does not protect against hippocampal damage after status epilepticus in mice." in: Cell death & disease, Vol. 8, Issue 1, pp. e2556, (2017) (PubMed).

Griesser, Vemula, Raulien, Wagner, Reeg, Grune, Fedorova: "Cross-talk between lipid and protein carbonylation in a dynamic cardiomyocyte model of mild nitroxidative stress." in: Redox biology, Vol. 11, pp. 438-455, (2017) (PubMed).

Chen, Fang, Su, Hsu, Yang, Huang: "Lapatinib induces autophagic cell death and differentiation in acute myeloblastic leukemia." in: OncoTargets and therapy, Vol. 9, pp. 4453-64, (2016) (PubMed).

Graham, Kaza, Klocke, Gillespie, Shevde, Carroll, Roth: "Tamoxifen Induces Cytotoxic Autophagy in Glioblastoma." in: Journal of neuropathology and experimental neurology, Vol. 75, Issue 10, pp. 946-954, (2016) (PubMed).

He, Yuan, Qi, Zhang, Wang: "Atorvastatin induces autophagic cell death in prostate cancer cells in vitro." in: Molecular medicine reports, Vol. 11, Issue 6, pp. 4403-8, (2015) (PubMed).

Vlahava, Eliopoulos, Sourvinos: "CD40 ligand exhibits a direct antiviral effect on Herpes Simplex Virus type-1 infection via a PI3K-dependent, autophagy-independent mechanism." in: Cellular signalling, Vol. 27, Issue 6, pp. 1253-63, (2015) (PubMed).

Hsin, Ou, Wu, Jan, Hsiao, Lin, Ko: "GMI, an Immunomodulatory Protein from Ganoderma microsporum, Potentiates Cisplatin-Induced Apoptosis via Autophagy in Lung Cancer Cells." in: Molecular pharmaceutics, Vol. 12, Issue 5, pp. 1534-43, (2015) (PubMed).

Rabionet, Bayerle, Jennemann, Heid, Fuchser, Marsching, Porubsky, Bolenz, Guillou, Gröne, Gorgas, Sandhoff: "Male meiotic cytokinesis requires ceramide synthase 3-dependent sphingolipids with unique membrane anchors." in: Human molecular genetics, Vol. 24, Issue 17, pp. 4792-808, (2015) (PubMed).

Kashima, Shintani-Ishida, Nakajima, Maeda, Unuma, Uchiyama, Yoshida: "Immunohistochemical study of the autophagy marker microtubule-associated protein 1 light chain 3 in normal and steatotic human livers." in: Hepatology research : the official journal of the Japan Society of Hepatology, Vol. 44, Issue 7, pp. 779-87, (2014) (PubMed).

Islam, Shin, Yun, Cho, Seo, Chae, Cho: "The effect of RNAi silencing of p62 using an osmotic polysorbitol transporter on autophagy and tumorigenesis in lungs of K-rasLA1 mice." in: Biomaterials, Vol. 35, Issue 5, pp. 1584-96, (2013) (PubMed).

Zhang, Morgan, Chen, Choksi, Liu: "Induction of autophagy is essential for monocyte-macrophage differentiation." in: Blood, Vol. 119, Issue 12, pp. 2895-905, (2012) (PubMed).

Zhou, Kang, Spector: "Production of interferon ? by human immunodeficiency virus type 1 in human plasmacytoid dendritic cells is dependent on induction of autophagy." in: The Journal of infectious diseases, Vol. 205, Issue 8, pp. 1258-67, (2012) (PubMed).

Xing, Zhang, Li, Zhang, Li, Dang, Li, Fan, Yu, Pei, Zeng: "Beclin 1 knockdown inhibits autophagic activation and prevents the secondary neurodegenerative damage in the ipsilateral thalamus following focal cerebral infarction." in: Autophagy, Vol. 8, Issue 1, pp. 63-76, (2012) (PubMed).

Song, Lee: "Mouse knock-out of IOP1 protein reveals its essential role in mammalian cytosolic iron-sulfur protein biogenesis." in: The Journal of biological chemistry, Vol. 286, Issue 18, pp. 15797-805, (2011) (PubMed).

Tuffy, Concannon, DOrsi, King, Woods, Huber, Ward, Prehn: "Characterization of Puma-dependent and Puma-independent neuronal cell death pathways following prolonged proteasomal inhibition." in: Molecular and cellular biology, Vol. 30, Issue 23, pp. 5484-501, (2010) (PubMed).

Rouschop, van den Beucken, Dubois, Niessen, Bussink, Savelkouls, Keulers, Mujcic, Landuyt, Voncken, Lambin, van der Kogel, Koritzinsky, Wouters: "The unfolded protein response protects human tumor cells during hypoxia through regulation of the autophagy genes MAP1LC3B and ATG5." in: The Journal of clinical investigation, Vol. 120, Issue 1, pp. 127-41, (2010) (PubMed).

Byun, Yoon, An, Park, Kang, Kim, Lee: "The Rac1/MKK7/JNK pathway signals upregulation of Atg5 and subsequent autophagic cell death in response to oncogenic Ras." in: Carcinogenesis, Vol. 30, Issue 11, pp. 1880-8, (2009) (PubMed).

Target Details for MAP1LC3A

Target: MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Alternative Name: LC3

Background: MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene.

Gene ID: 84557

NCBI Accession: NP_115903, NP_852610

UniProt: Q9H492, Q9GZQ8

Pathways: Autophagy