LC3 (APG8) antibody

Antigen: LC3 (APG8)

Clonality: Monoclonal (166AT1234)

Host: Mouse

Reactivity: Human, Rat (Rattus)

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN387792

Additional Information

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Gene ID: 84557

Swiss-Prot: Q9H492

Immunogen: This LC3 antibody is generated from mouse immunized with a full length recombinant protein of human LC3 (APG8).

Isotype: IgG1 kappa  (Matching secondary antibodies)

Clone: 166AT1234

Description: Other names: MAP1LC3A, microtubule-associated protein 1 light chain 3 alpha, RP11-346K17.1, LC3, LC3A, MAP1ALC3, MAP1BLC3, MAP1 light chain 3-like protein 1, MAP1A/1B light chain 3 A, MAP1A/MAP1B LC3 A, MAP1A/MAP1B light chain 3 A, OTTHUMP00000030698, autophagy-relate

Specificity: This antibody is generated from mouse immunized with a full length recombinant protein of human LC3 (APG8).

Molecular Weight: 14272 DA

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:25~100 Immunocytochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Cell Signaling, Cell Structure, Autophagy

Restrictions: For Research Use only

Images

(TOP LEFT)Western blot analysis of anti-LC3 Mab (Cat. #ABIN387792) at 8 ug/ml. Lane 1: Y79 (soluble fraction of cell extract), Lane 2: 293 transfected with human LC3 (whole cell extract). (TOP RIGHT)Mouse cerebellar cell lines stably expressing human GFP-LC3 fusion protein were evaluated by immunocytochemistry using the anti-LC3 monoclonal antibody ABIN387792. Punctuate autophagic cell vesicles , with numerous GFP-LC3 vesicles are displayed. Conditions: 1:1 methanol:acetone fixation, 0.2% saponin permeabilization. (BOTTOM) Western blot analysis of anti-LC3 Mab (Cat. #ABIN387792) Hela cell lysates, which were treated with rapamycin or bafilomycin overnight. Data courtesy of Dr. David Rubinsztein, Cambridge Institute for Medical Research. (TOP) 10X( lower panel) and 20X (upper panel) immunohistochemistry images from muscle tissue of a diseased mouse off Dox after 5 weeks on regular food. Several fibers that have autophagic vesicles throughout are visible. Primary antibody used is Cat# ABIN387792. Data courtesy of Dr. Christy Caudill, Cincinnati Children's Hospital Medical Center. (MIDDLE) Cathepsin D WT vs KO (postnatal day 25) mouse thalamus exhibiting clumped immunoreactivity for LC3 in the KO brain (arrows, upper panel) and relative absence of clumped LC3 immunoreactivity in the WT control (staining is diffuse and cytoplasmic, lower panel). Data courtesy of Dr. John Shacka, Neuropathology Division, University of Alabama at Birmingham. (BOTTOM) Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with Autophagy LC3 (APG8) antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry, clinical relevance has not been evaluated.

anti-LC3 (APG8) antibody (Image 2)

Publications

Publications: Baras, Moskaluk: "Intracellular localization of GASP/ECOP/VOPP1." in: Journal of molecular histology, 2010 (PubMed).