LC3 (APG8) antibody

Antigen: LC3 (APG8)

Clonality: Monoclonal (166AT1234)

Host: Mouse

Reactivity: Human, Rat (Rattus)

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN387792

Additional Information

Gene ID: 84557

UniProt: Q9H492

Immunogen: This LC3 antibody is generated from mouse immunized with a full length recombinant protein of human LC3 (APG8).

Isotype: IgG1 kappa

Clone: 166AT1234

Description: Other names: MAP1LC3A, microtubule-associated protein 1 light chain 3 alpha, RP11-346K17.1, LC3, LC3A, MAP1ALC3, MAP1BLC3, MAP1 light chain 3-like protein 1, MAP1A/1B light chain 3 A, MAP1A/MAP1B LC3 A, MAP1A/MAP1B light chain 3 A, OTTHUMP00000030698, autophagy-relate

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: This LC3 antibody is generated from mouse immunized with a full length recombinant protein of human LC3 (APG8).

Molecular Weight: 14272 DA

Comments:

Background: MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:25~100 Immunocytochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Cell Signaling, Cell Structure, Autophagy

Restrictions: For Research Use only

Images

(TOP LEFT)Western blot analysis of anti-LC3 Mab (Cat. #ABIN387792) at 8 ug/ml. Lane 1: Y79 (soluble fraction of cell extract), Lane 2: 293 transfected with human LC3 (whole cell extract). (TOP RIGHT)Mouse cerebellar cell lines stably expressing human GFP-LC3 fusion protein were evaluated by immunocytochemistry using the anti-LC3 monoclonal antibody ABIN387792. Punctuate autophagic cell vesicles , with numerous GFP-LC3 vesicles are displayed. Conditions: 1:1 methanol:acetone fixation, 0.2% saponin permeabilization. (BOTTOM) Western blot analysis of anti-LC3 Mab (Cat. #ABIN387792) Hela cell lysates, which were treated with rapamycin or bafilomycin overnight. Data courtesy of Dr. David Rubinsztein, Cambridge Institute for Medical Research. (TOP) 10X( lower panel) and 20X (upper panel) immunohistochemistry images from muscle tissue of a diseased mouse off Dox after 5 weeks on regular food. Several fibers that have autophagic vesicles throughout are visible. Primary antibody used is Cat# ABIN387792. Data courtesy of Dr. Christy Caudill, Cincinnati Children's Hospital Medical Center. (MIDDLE) Cathepsin D WT vs KO (postnatal day 25) mouse thalamus exhibiting clumped immunoreactivity for LC3 in the KO brain (arrows, upper panel) and relative absence of clumped LC3 immunoreactivity in the WT control (staining is diffuse and cytoplasmic, lower panel). Data courtesy of Dr. John Shacka, Neuropathology Division, University of Alabama at Birmingham. (BOTTOM) Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with Autophagy LC3 (APG8) antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry, clinical relevance has not been evaluated.

anti-LC3 (APG8) antibody (Image 2)

Publications

Baras, Moskaluk: "Intracellular localization of GASP/ECOP/VOPP1." in: Journal of molecular histology, 2010 (PubMed).

Product: Shen, Kepp, Martins et al.: "Defective autophagy associated with LC3 puncta in epothilone-resistant cancer cells." in: Cell cycle (Georgetown, Tex.), Vol. 9, Issue 2, pp. 377-83, 2010 (PubMed).

Gao, Gammoh, Wong et al.: "Processing of autophagic protein LC3 by the 20S proteasome." in: Autophagy, Vol. 6, Issue 1, pp. 126-37, 2010 (PubMed).

Jiang, Cheng, Liu et al.: "Protein kinase C inhibits autophagy and phosphorylates LC3." in: Biochemical and biophysical research communications, Vol. 395, Issue 4, pp. 471-6, 2010 (PubMed).

Namgoong, Khanal, Cho et al.: "The prolyl isomerase Pin1 induces LC-3 expression and mediates tamoxifen resistance in breast cancer." in: The Journal of biological chemistry, Vol. 285, Issue 31, pp. 23829-41, 2010 (PubMed).

Gao, Cao, Bao et al.: "Autophagy negatively regulates Wnt signalling by promoting Dishevelled degradation." in: Nature cell biology, Vol. 12, Issue 8, pp. 781-90, 2010 (PubMed).