|+1 404 474 4654|
|+1 888 205 9894 (TF)|
LC3 (APG8) antibody
Human, Rat (Rattus)
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA
|6 references available|
|Quantity||0.1 mg (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This LC3 antibody is generated from mouse immunized with a full length recombinant protein of human LC3 (APG8).|
|Description||Other names: MAP1LC3A, microtubule-associated protein 1 light chain 3 alpha, RP11-346K17.1, LC3, LC3A, MAP1ALC3, MAP1BLC3, MAP1 light chain 3-like protein 1, MAP1A/1B light chain 3 A, MAP1A/MAP1B LC3 A, MAP1A/MAP1B light chain 3 A, OTTHUMP00000030698, autophagy-relate|
|Characteristics||Purified Mouse Monoclonal Antibody (Mab)|
|Specificity||This LC3 antibody is generated from mouse immunized with a full length recombinant protein of human LC3 (APG8).|
|Molecular Weight||14272 DA|
Background: MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene.
|Application Notes||The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:25~100 Immunocytochemistry ~~ 1:50~100|
|Buffer||Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca|
|Research Area||Cell Signaling, Cell Structure, Autophagy|
|Restrictions||For Research Use only|
Baras, Moskaluk: "Intracellular localization of GASP/ECOP/VOPP1." in: Journal of molecular histology, 2010 (PubMed).
Shen, Kepp, Martins et al.: "Defective autophagy associated with LC3 puncta in epothilone-resistant cancer cells." in: Cell cycle (Georgetown, Tex.), Vol. 9, Issue 2, pp. 377-83, 2010 (PubMed).
Gao, Gammoh, Wong et al.: "Processing of autophagic protein LC3 by the 20S proteasome." in: Autophagy, Vol. 6, Issue 1, pp. 126-37, 2010 (PubMed).
Jiang, Cheng, Liu et al.: "Protein kinase C inhibits autophagy and phosphorylates LC3." in: Biochemical and biophysical research communications, Vol. 395, Issue 4, pp. 471-6, 2010 (PubMed).
Namgoong, Khanal, Cho et al.: "The prolyl isomerase Pin1 induces LC-3 expression and mediates tamoxifen resistance in breast cancer." in: The Journal of biological chemistry, Vol. 285, Issue 31, pp. 23829-41, 2010 (PubMed).
Gao, Cao, Bao et al.: "Autophagy negatively regulates Wnt signalling by promoting Dishevelled degradation." in: Nature cell biology, Vol. 12, Issue 8, pp. 781-90, 2010 (PubMed).