Euchromatic Histone-Lysine N-Methyltransferase 1 (EHMT1) (Center) antibody

Antigen: Euchromatic Histone-Lysine N-Methyltransferase 1 (EHMT1)

Synonyms: GLP, KMT1D, DEL9q34, FP13812, FLJ12879, KIAA1876, EUHMTASE1, Eu-HMTase1, bA188C12.1, DKFZp667M072, RP11-188C12.1, EHMT1, MGC157157, G9a, NV11612, ehmt1, MGC122333

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN387856

Additional Information

Alternative name: EHMT1

Gene ID: 79813

UniProt: Q9H9B1

Immunogen: This EHMT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 343~373 amino acids from the Center region of human EHMT1.

Isotype: Ig

Description: Other names: Histone H3-K9 methyltransferase 5, H3-K9-HMTase 5, Euchromatic histone-lysine N-methyltransferase 1, Eu-HMTase1, G9a-like protein 1, GLP1, Lysine N-methyltransferase 1D, EHMT1, EUHMTASE1, KIAA1876, KMT1D

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This EHMT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 343~373 amino acids from the Center region of human EHMT1.

Molecular Weight: 138254 DA

Comments:

Background: EHMT1, also known as EUHMTASE1, is a histone methyltransferase. This protein methylates 'Lys-9' of histone H3 in vitro. H3 'Lys-9' methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. EHMT1 is Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in the G0/G1 transition of the cell cycle.

Application Details

Application Notes: The suggested dilution is: ELISA1:1,000 Western blotting 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications, Cell Cycle, Transcription Factors, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of EHMT1 (arrow) using rabbit polyclonal EHMT1 Antibody (Center)(ABIN387856). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EHMT1 gene (Lane 2) (Origene Technologies).

Publications

Product: Ogawa, Ishiguro, Gaubatz et al.: "A complex with chromatin modifiers that occupies E2F- and Myc-responsive genes in G0 cells." in: Science (New York, N.Y.), Vol. 296, Issue 5570, pp. 1132-6, 2002 (PubMed).

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).