DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B) antibody

Antigen: DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B)

Synonyms: cb633, dnmt3b, MGC152725, DNMT3B, LOC100218113, ICF, M.HsaIIIB

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN387884

Additional Information

Alternative name: Dnmt3b

Gene ID: 1789

UniProt: Q9UBC3

Immunogen: This Dnmt3b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 395~425 amino acids from the center region of human Dnmt3b.

Isotype: Ig

Description: Other names: DNA (cytosine-5)-methyltransferase 3B, Dnmt3b, DNA methyltransferase HsaIIIB, DNA MTase HsaIIIB, M.HsaIIIB

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Dnmt3b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 395~425 amino acids from the center region of human Dnmt3b.

Molecular Weight: 95751 DA

Comments:

Background: CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. Dnmt3b is a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF)syndrome.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications, Cancer, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-Dnmt3b Pab (ABIN387884) in T47-D cell lysate. Dnmt3b (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with Dnmt3b antibody (ABIN387884), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Lu, Markowetz, Unwin et al.: "Systems-level dynamic analyses of fate change in murine embryonic stem cells." in: Nature, Vol. 462, Issue 7271, pp. 358-62, 2009 (PubMed).

Product: Okano, Bell, Haber et al.: "DNA methyltransferases Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development." in: Cell, Vol. 99, Issue 3, pp. 247-57, 1999 (PubMed).