DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B) antibody

Antigen: DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B)

Synonyms: cb633, dnmt3b, MGC152725, DNMT3B, LOC100218113, ICF, M.HsaIIIB

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN387884

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: Dnmt3b

Gene ID: 1789

Swiss-Prot: Q9UBC3

Immunogen: This Dnmt3b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 395~425 amino acids from the center region of human Dnmt3b.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: DNA (cytosine-5)-methyltransferase 3B, Dnmt3b, DNA methyltransferase HsaIIIB, DNA MTase HsaIIIB, M.HsaIIIB

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human Dnmt3b.

Molecular Weight: 95751 DA

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications, Cancer, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-Dnmt3b Pab (ABIN387884) in T47-D cell lysate. Dnmt3b (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with Dnmt3b antibody (ABIN387884), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Publications: Lu, Markowetz, Unwin et al.: "Systems-level dynamic analyses of fate change in murine embryonic stem cells." in: Nature, Vol. 462, Issue 7271, pp. 358-62, 2009 (PubMed).