SET Domain, Bifurcated 1 (SETDB1) (C-Term) antibody

Antigen: SET Domain, Bifurcated 1 (SETDB1)

Synonyms: ESET, KG1T, KMT1E, KIAA0067, H3-K9-HMTase4, AU022152, MGC90670, mKIAA0067, SETDB1, NV10069, fc07d03, wu:fc07d03, si:ch211-81a5.6

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN387924

Additional Information

Alternative name: SETDB1

Gene ID: 9869

UniProt: Q15047

Immunogen: This SETDB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1202~1232 amino acids from the C-terminal region of human SETDB1.

Isotype: Ig

Description: Other names: Histone H3-K9 methyltransferase 4, H3-K9-HMTase 4, SET domain bifurcated 1, ERG-associated protein with SET domain, ESET,, KMT1E

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This SETDB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1202~1232 amino acids from the C-terminal region of human SETDB1.

Molecular Weight: 143157 DA

Comments:

Background: The SET domain is a highly conserved, approximately 150-amino acid motif implicated in the modulation of chromatin structure. It was originally identified as part of a larger conserved region present in the Drosophila Trithorax protein and was subsequently identified in the Drosophila Su(var)3-9 and 'Enhancer of zeste' proteins, from which the acronym SET is derived. Studies have suggested that the SET domain may be a signature of proteins that modulate transcriptionally active or repressed chromatin states through chromatin remodeling activities.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications, Cancer, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-SETDB1 Pab(ABIN387924) in Ramos cell line lysate (35ug/lane). SETDB1(arrow) was detected using the purified Pab (1:60 dilution).

Western blot analysis of SETDB1(arrow) using rabbit polyclonal SETDB1 Antibody (C-term) (ABIN387924). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SETDB1 gene (Lane 2) (Origene Technologies).

Publications

Product: Schultz, Ayyanathan, Negorev et al.: "SETDB1: a novel KAP-1-associated histone H3, lysine 9-specific methyltransferase that contributes to HP1-mediated silencing of euchromatic genes by KRAB zinc-finger proteins." in: Genes & development, Vol. 16, Issue 8, pp. 919-32, 2002 (PubMed).

Wang, An, Cao et al.: "mAM facilitates conversion by ESET of dimethyl to trimethyl lysine 9 of histone H3 to cause transcriptional repression." in: Molecular cell, Vol. 12, Issue 2, pp. 475-87, 2003 (PubMed).

Sarraf, Stancheva: "Methyl-CpG binding protein MBD1 couples histone H3 methylation at lysine 9 by SETDB1 to DNA replication and chromatin assembly." in: Molecular cell, Vol. 15, Issue 4, pp. 595-605, 2004 (PubMed).

Ichimura, Watanabe, Sakamoto et al.: "Transcriptional repression and heterochromatin formation by MBD1 and MCAF/AM family proteins." in: The Journal of biological chemistry, Vol. 280, Issue 14, pp. 13928-35, 2005 (PubMed).