Histone Deacetylase 9 (HDAC9) (C-Term) antibody

Antigen: Histone Deacetylase 9 (HDAC9)

Synonyms: HD7, HDAC, HDRP, MITR, HDAC7, HDAC7B, HDAC9B, HDAC9FL, KIAA0744, DKFZp779K1053, HD9, HD7B, Mitr, Hdac7b, AV022454, mKIAA0744, D030072B18Rik, RGD1310748, hdac9, MGC73392, zgc:73392, HDAC9, TWIST, DKFZp459E171, HDA09, HDA9, HISTONE DEACETYLASE, HISTONE DEACETYLASE 9

Epitope: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN387961

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: HDAC9 (C-term)

Gene ID: 9734

Swiss-Prot: Q9UKV0

Immunogen: This HDAC9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 511~540 amino acids from the C-terminal region of human HDAC9.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: Histone deacetylase 9, HD9, HD7B

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human HDAC9.

Molecular Weight: 111297 DA

Synonyms: HD7, HDAC, HDRP, MITR, HDAC7, HDAC7B, HDAC9B, HDAC9FL, KIAA0744, DKFZp779K1053, HD9, HD7B, Mitr, Hdac7b, AV022454, mKIAA0744, D030072B18Rik, RGD1310748, hdac9, MGC73392, zgc:73392, HDAC9, TWIST, DKFZp459E171, HDA09, HDA9, HISTONE DEACETYLASE, HISTONE DEACETYLASE 9

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100 Immunoprecipitation~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications, Cell Cycle, Transcription Factors, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

Both anti-HDAC9 N-term (ABIN387960) and C-term (ABIN387961) Pab were tested by WB and IP-WB using HeLa and HeLa-HDAC9 transfected cells. Top figure shows both Pab specifically detect HDAC9 in HeLa-HDAC9 transfected cell but not HeLa alone. Bottom figure shows that both Pab can immunoprecipitate (IP) HDAC9 from HeLa-HDAC9 tranfected cells. (Data kindly provided by Dr. Zhigang Yuan, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.