Ubiquitin-Like Modifier (ISG15) (N-Term) antibody

Antigen: Ubiquitin-Like Modifier (ISG15)

Synonyms: G1P2, UCRP, IFI15, G1p2, IP17, Irfp, IGI15, MGC18616, MGC103144, MGC130321

Epitope: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN387983

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: ISG15 (N-term)

Gene ID: 9636

Swiss-Prot: P05161

Immunogen: This ISG15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39~68 amino acids from the N-terminal region of human ISG15.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: Interferon-induced 17 kDa protein, Ubiquitin cross-reactive protein, hUCRP, Interferon-induced 15 kDa protein,G1P2, UCRP

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human ISG15.

Molecular Weight: 17756 DA

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Ubiquitin-related antibodies, Protein Modifications, Cancer, Cell Cycle, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Western blot analysis of ISG15 (arrow) using rabbit polyclonal hISG15-A46 (ABIN387983). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ISG15 gene (Lane 2) .

ISG15 Antibody (A46) (ABIN387983) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the ISG15 antibody detected the ISG15 protein (arrow).