Ubiquitin (C-Term) antibody

Antigen: Ubiquitin

Synonyms: MGC8385, FLJ25987, Ubc, Ubb2, Uba52, Rps27a, AL033289, CG11624, DmelCG11624, DmUb, Ub, ubi, Ubi-p, ubi-p63E, Ubi-P63e, ubi63E, Ubi63E, Ubi63p, Ubip, Ubiq, Ubp, UBC, CG18282, CG32744, CR32744, DmelCG32744, ubiquitin, si:dz180g5.5, si:busm1-180g5.5, UBB, Ubiquitin, CG11700, ESTS:143G11T, ESTS:199A6T, anon-sts41, poly-ub, DmelCR11700, LOC100194618, UBIQ, LOC100282068

Epitope: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388040

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: Ubiquitin (C-term)

Gene ID: 7314

Swiss-Prot: P62988

Immunogen: This Ubiquitin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 44~73 amino acids from the C-terminal region of human Ubiquitin.

Isotype: Ig  (Matching secondary antibodies)

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human Ubiquitin.

Molecular Weight: 8565 DA

Synonyms: MGC8385, FLJ25987, Ubc, Ubb2, Uba52, Rps27a, AL033289, CG11624, DmelCG11624, DmUb, Ub, ubi, Ubi-p, ubi-p63E, Ubi-P63e, ubi63E, Ubi63E, Ubi63p, Ubip, Ubiq, Ubp, UBC, CG18282, CG32744, CR32744, DmelCG32744, ubiquitin, si:dz180g5.5, si:busm1-180g5.5, UBB, Ubiquitin, CG11700, ESTS:143G11T, ESTS:199A6T, anon-sts41, poly-ub, DmelCR11700, LOC100194618, UBIQ, LOC100282068

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100 Immunofluorescence

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Ubiquitin-related antibodies, Protein Modifications, Signaling, Chromatin, Neurology, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-Ubiquitin Pab (ABIN388040) in HeLa cell lysate. Ubiquitin (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Characterization of FOXP2 Isoforms. FOXP2.10t was detected with an antibody to the N-terminal XpressTM tag or FOXP2 (green) and counterstained with antibodies to the aggresome marker ubiquitin (red). Nuclei are marked by DAPI staining (blue). Ubiquitin co-localizes with FOXP2.10t aggregates suggesting that these cellular bodies represent aggresomes. (Hum. Mol. Genet. 2006 Nov 01.15(21):3154-3167)