SUMO1/sentrin Specific Protease 6 (SENP6) (C-Term) antibody

Antigen: SUMO1/sentrin Specific Protease 6 (SENP6)

Synonyms: SSP1, SUSP1, FLJ11355, FLJ11887, KIAA0389, KIAA0797

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388052

Additional Information

Alternative name: SENP6

Gene ID: 26054

UniProt: Q9GZR1

Immunogen: This SENP6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1083~1112 amino acids from the C-terminal region of human SENP6.

Isotype: Ig

Description: Other names: Sentrin-specific protease 6, Sentrin/SUMO-specific protease SENP6, SUMO-1-specific protease 1, SENP6, KIAA0797, SSP1, SUSP1

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This SENP6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1083~1112 amino acids from the C-terminal region of human SENP6.

Molecular Weight: 126144 DA

Comments:

Background: SENP6 (SUSP1) is a protease that deconjugates SUMO1, SUMO2 and SUMO3 from targeted proteins. This protein does not seem to be involved in the processing of full-length SUMO proteins to their mature conjugatable forms. SENP6 deconjugates SUMO1 from RXRA, leading to transcriptional activation. It may act preferentially on substrates containing 3 or more SUMO2 or SUMO3 moieties.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Apoptosis/Necrosis, Cell Signaling, Protein Modifications, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of SENP6 polyclonal antibody (ABIN388052) in HL60 cell lysate. SENP6 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Kim, Baek, Jeon et al.: "A new SUMO-1-specific protease, SUSP1, that is highly expressed in reproductive organs." in: The Journal of biological chemistry, Vol. 275, Issue 19, pp. 14102-6, 2000 (PubMed).

Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).