Protein Inhibitor of Activated STAT, 2 (PIAS2) (C-Term), (AA 518-547) antibody

Details for Product No. ABIN388059
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Antigen
Synonyms MGC83751, piasx, wu:fi05f09, PIAS2, 6330408K17Rik, AI462206, ARIP3, AU018068, Dib, Miz1, PIASxalpha, PIASxb, PIASxbeta, SIZ2, DIP, MIZ, MIZ1, PIASX, PIASX-ALPHA, PIASX-BETA, ZMIZ4
Epitope
C-Term, AA 518-547
(19), (16), (10), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(71), (35), (26), (9), (2), (1), (1)
Host
Rabbit
(62), (11), (2), (1)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC)
(69), (40), (18), (14), (4), (3), (3), (3), (2), (2), (1), (1)
Pubmed 4 references available
Quantity 400 μL
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Catalog No. ABIN388059
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Immunogen This PIAS2 (PIASx1/2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 518-547 AA from the C-terminal region of human PIAS2 (PIASx1/2).
Clone RB0701
Isotype Ig
Specificity This PIAS2 (PIASx1/2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 525~555 amino acids from the C-terminal region of human PIASx1/2.
Predicted Reactivity Rat (Rattus)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name PIAS2 (PIASx1/2)
Background PIASX functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. This protein plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and the PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing. PIASX binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIAS2-beta, but not isoform PIAS2-alpha, promotes MDM2 sumoylation. Isoform PIAS2-alpha promotes PARK7 sumoylation. Isoform PIAS2-beta promotes NCOA2 sumoylation more efficiently than isoform PIAS2-alpha.
Synonyms: E3 SUMO-protein ligase PIAS2, Protein inhibitor of activated STAT2, Protein inhibitor of activated STAT x, Msx-interacting zinc finger protein, Miz1, DAB2-interacting protein, DIP, Androgen receptor-interacting protein 3, ARIP3, PIAS-NY protein, PIAS2, PI
Molecular Weight 68254 DA
Gene ID 9063
UniProt O75928
Research Area Apoptosis/Necrosis, Cell Signaling, Protein Modifications, Transcription Factors, Signaling, Cell Structure
Application Notes IHC = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 1.36 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Protein Inhibitor of Activated STAT, 2 (PIAS2) (C-Term), (AA 518-547) antibody Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
Background publications Liu, Liao, Rao et al.: "Inhibition of Stat1-mediated gene activation by PIAS1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 18, pp. 10626-31, 1998 (PubMed).

Wu, Cetinkaya, Munoz-Alonso et al.: "Myc represses differentiation-induced p21CIP1 expression via Miz-1-dependent interaction with the p21 core promoter." in: Oncogene, Vol. 22, Issue 3, pp. 351-60, 2003 (PubMed).

Arora, Liu, He et al.: "PIASx is a transcriptional co-repressor of signal transducer and activator of transcription 4." in: The Journal of biological chemistry, Vol. 278, Issue 24, pp. 21327-30, 2003 (PubMed).

Sakurai, Itoh, Higashitsuji et al.: "A cleaved form of MAGE-A4 binds to Miz-1 and induces apoptosis in human cells." in: The Journal of biological chemistry, Vol. 279, Issue 15, pp. 15505-14, 2004 (PubMed).

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