PIAS2 antibody (C-Term)

Catalog No. ABIN2779873

This Rabbit Polyclonal antibody specifically detects PIAS2 in WB and IHC. It exhibits reactivity toward Human, Mouse, Dog, Cow, Horse and Rabbit.

Quick Overview for PIAS2 antibody (C-Term) (ABIN2779873)

Target: PIAS2 (Protein Inhibitor of Activated STAT, 2 (PIAS2))

Reactivity: Human, Mouse, Dog, Cow, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIAS2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-PIAS2 Antibody

Binding Specificity: C-Term

Purpose: Pias2 Antibody - C-terminal region

Sequence: LSLIPVDPQY CPPMFLDSLT SPLTASSTSV TTTSPHESST HVSSSSSRSE

Predicted Reactivity: Cow: 93%, Dog: 100%, Horse: 100%, Human: 93%, Mouse: 100%, Rabbit: 93%

Characteristics: This is a rabbit polyclonal antibody against Pias2. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of mouse Pias2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8109279

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for PIAS2

Target: PIAS2 (Protein Inhibitor of Activated STAT, 2 (PIAS2))

Alternative Name: Pias2

Background:

Background Information: Pias2 functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. It plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. It seems to be mostly involved in gene silencing. It binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity.

Gene Name: Protein inhibitor of activated STAT 2

Alternative Symbols: Di, PI, DIP, Dib, Miz, Miz1, PIAS, SIZ2, ARIP3, PIASxb, AI462206, AU018068, PIASxbeta, PIASxalpha, PIASxalpha6

Protein Name: E3 SUMO-protein ligase PIAS2

Molecular Weight: 54kDa

Gene ID: 17344

UniProt: Q8C5D8

Pathways: JAK-STAT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling