PIAS2 antibody (C-Term)

Catalog No. ABIN1882114

This Rabbit Polyclonal antibody specifically detects PIAS2 in WB and IHC (p). It exhibits reactivity toward Human and has been mentioned in 4+ publications.

Quick Overview for PIAS2 antibody (C-Term) (ABIN1882114)

Target: PIAS2 (Protein Inhibitor of Activated STAT, 2 (PIAS2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIAS2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB0676

Product Details anti-PIAS2 Antibody

Binding Specificity: AA 1-30, C-Term

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This PIAS2 (PIASx1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the C-terminal region of human PIAS2 (PIASx1).

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-PIAS2 antibody (ABIN1882114)

Jones, Fusi, Higham, Abdel-Hafiz, Horwitz, Lam, Brosens: "Regulation of the SUMO pathway sensitizes differentiating human endometrial stromal cells to progesterone." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 44, pp. 16272-7, (2006) (PubMed).

Arora, Liu, He, Kim, Murphy, Murphy, Modlin, Shuai: "PIASx is a transcriptional co-repressor of signal transducer and activator of transcription 4." in: The Journal of biological chemistry, Vol. 278, Issue 24, pp. 21327-30, (2003) (PubMed).

Wu, Cetinkaya, Munoz-Alonso, von der Lehr, Bahram, Beuger, Eilers, Leon, Larsson: "Myc represses differentiation-induced p21CIP1 expression via Miz-1-dependent interaction with the p21 core promoter." in: Oncogene, Vol. 22, Issue 3, pp. 351-60, (2003) (PubMed).

Liu, Liao, Rao, Kushner, Chung, Chang, Shuai: "Inhibition of Stat1-mediated gene activation by PIAS1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 18, pp. 10626-31, (1998) (PubMed).

Target Details for PIAS2

Target: PIAS2 (Protein Inhibitor of Activated STAT, 2 (PIAS2))

Background: PIASX functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. This protein plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and the PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing. PIASX binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. This antibody is directed against an epitope specific to PIAS2-alpha. Isoform PIAS2-beta, but not isoform PIAS2-alpha, promotes MDM2 sumoylation. Isoform PIAS2-alpha promotes PARK7 sumoylation. Isoform PIAS2-beta promotes NCOA2 sumoylation more efficiently than isoform PIAS2-alpha.

Molecular Weight: 68240

NCBI Accession: NP_004662, NP_775298

UniProt: O75928

Pathways: JAK-STAT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling