BCL2-Related Ovarian Killer (BOK) antibody

Antigen: BCL2-Related Ovarian Killer (BOK)

Synonyms: BOKL, BCL2L9, MGC4631, BOK, MGC64041, zgc:64041, bok, zgc:100979, MGC159657

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388103

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: Bok BH3 Domain

Gene ID: 666

Swiss-Prot: Q9UMX3

Immunogen: This Bok BH3 Domain antibody was developed against a synthetic peptide selected from the N-terminal region (BH3 domain) of human Bok.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: Bcl-2-related ovarian killer protein, Hbok

Specificity: This antibody was developed against a synthetic peptide selected from the N-terminal region (BH3 domain) of human Bok.

Molecular Weight: 23540 DA

Application Details

Application Notes: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Apoptosis/Necrosis, Cancer, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-Bok BH3 domain Pab (ABIN388103) in HL-60 cell lysate. Bok BH3 domain (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.