BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) (BH3 Domain) antibody

Antigen: BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3)

Synonyms: NIP3

Binding Site: BH3 Domain

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388117

Additional Information

Alternative name: BNIP3 (NIP3)

Gene ID: 664

UniProt: Q12983

Immunogen: This BNIP3 (NIP3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region (BH3 domain) of human NIP3.

Isotype: Ig

Description: Other names: BCL2/adenovirus E1B 19-kDa protein-interacting protein 3, BNIP3

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This BNIP3 Antibody antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region (BH3 domain) of human NIP3.

Molecular Weight: 21541 DA

Comments:

Background: NIP3 is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein which is responsible for the protection of virally-induced cell death, as well as E1B 19 kDa-like sequences of BCL2, also an apoptotic protector. NIP3 contains a BH3 domain and a transmembrane domain, which have been associated with pro-apoptotic function. The dimeric mitochondrial protein is known to induce apoptosis, even in the presence of BCL2.

Application Details

Application Notes: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100 Immunofluorescence~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Apoptosis/Necrosis, Cell Structure, Cancer

Restrictions: For Research Use only

Images

The anti-NIP3 BH3 domain Pab (ABIN388117) is used in Western blot to detect NIP3 BH3 in Ramos cell lysate (lane 1) and in mouse brain tissue lysate (lane 2).

Freshly isolated mouse hepatocytes plated on coverslips (2 x105 cells/22-mm glass coverslip) were cultured under normoxic conditions for 6 hr. The cells were then fixed in 2% paraformaldehyde in PBS for 1 hr, and processed for confocal immunofluorescence (red: F-actin, blue: ATP-synthase, green: BNIP3). Fluorescence labeling of BNIP3 accomplished with anti-BNIP3 antibody ABIN388117. Data courtesy of Ruben Zamora, University of Pittsburgh.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Yasuda, Theodorakis, Subramanian et al.: "Adenovirus E1B-19K/BCL-2 interacting protein BNIP3 contains a BH3 domain and a mitochondrial targeting sequence." in: The Journal of biological chemistry, Vol. 273, Issue 20, pp. 12415-21, 1998 (PubMed).

Kothari, Cizeau, McMillan-Ward et al.: "BNIP3 plays a role in hypoxic cell death in human epithelial cells that is inhibited by growth factors EGF and IGF." in: Oncogene, Vol. 22, Issue 30, pp. 4734-44, 2003 (PubMed).