|+1 404 474 4654|
|+1 888 205 9894 (TF)|
BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) (BH3 Domain) antibody
Alternatives Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA
|2 references available|
|Quantity||400 µL (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Alternative name||BNIP3 (NIP3)|
|Immunogen||This BNIP3 (NIP3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region (BH3 domain) of human NIP3.|
|Description||Other names: BCL2/adenovirus E1B 19-kDa protein-interacting protein 3, BNIP3|
|Characteristics||Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This BNIP3 Antibody antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region (BH3 domain) of human NIP3.|
|Molecular Weight||21541 DA|
Background: NIP3 is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein which is responsible for the protection of virally-induced cell death, as well as E1B 19 kDa-like sequences of BCL2, also an apoptotic protector. NIP3 contains a BH3 domain and a transmembrane domain, which have been associated with pro-apoptotic function. The dimeric mitochondrial protein is known to induce apoptosis, even in the presence of BCL2.
|Application Notes||ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100 Immunofluorescence~~1:50~100|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Apoptosis/Necrosis, Cell Structure, Cancer|
|Restrictions||For Research Use only|
Yasuda, Theodorakis, Subramanian et al.: "Adenovirus E1B-19K/BCL-2 interacting protein BNIP3 contains a BH3 domain and a mitochondrial targeting sequence." in: The Journal of biological chemistry, Vol. 273, Issue 20, pp. 12415-21, 1998 (PubMed).
Kothari, Cizeau, McMillan-Ward et al.: "BNIP3 plays a role in hypoxic cell death in human epithelial cells that is inhibited by growth factors EGF and IGF." in: Oncogene, Vol. 22, Issue 30, pp. 4734-44, 2003 (PubMed).