HtrA Serine Peptidase 1 (HTRA1) (N-Term) antibody

Antigen: HtrA Serine Peptidase 1 (HTRA1)

Synonyms: PRSS11, L56, HtrA, ARMD7, ORF480, HTRA, Prss11, RSPP11, AI429470, MGC93339, htra1, zgc:92029, Xhtra1, zgc:172061, l56, htra, prss11, MGC145723

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388127

Additional Information

Alternative name: HtrA1

Gene ID: 5654

UniProt: Q92743

Immunogen: This HtrA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 124~153 amino acids from the N-terminal region of human HtrA1.

Isotype: Ig

Description: Other names: Serine protease HTRA1, L56

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This HtrA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 124~153 amino acids from the N-terminal region of human HtrA1.

Molecular Weight: 51286 DA

Comments:

Background: HtrA1 a member of the trypsin family of serine proteases. This protein is a secreted enzyme that is proposed to regulate the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. It has also been suggested to be a regulator of cell growth.

Application Details

Application Notes: The suggested dilution will be: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Apoptosis/Necrosis, Cancer, Cell Structure

Restrictions: For Research Use only

Images

The anti-HtrA1 N-term Pab (ABIN388127) is used in Western blot to detect HtrA1in mouse brain tissue lysate.

HtrA1 Antibody (N-term) (ABIN388127)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HtrA1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Publications

Product: Zumbrunn, Trueb: "Localization of the gene for a serine protease with IGF-binding domain (PRSS11) to human chromosome 10q25.3-q26.2." in: Genomics, Vol. 45, Issue 2, pp. 461-2, 1998 (PubMed).

Hu, Carozza, Klein et al.: "Human HtrA, an evolutionarily conserved serine protease identified as a differentially expressed gene product in osteoarthritic cartilage." in: The Journal of biological chemistry, Vol. 273, Issue 51, pp. 34406-12, 1999 (PubMed).

Chien, Staub, Hu et al.: "A candidate tumor suppressor HtrA1 is downregulated in ovarian cancer." in: Oncogene, Vol. 23, Issue 8, pp. 1636-44, 2004 (PubMed).

Howes, Lerch, Greenhalf et al.: "Clinical and genetic characteristics of hereditary pancreatitis in Europe." in: Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association, Vol. 2, Issue 3, pp. 252-61, 2004 (PubMed).