APOBEC3B (PHO3) (C-Term) antibody

Antigen: APOBEC3B (PHO3)

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388145

Additional Information

Gene ID: 9582

UniProt: Q9UH17

Immunogen: This PHO3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 307~337 amino acids from the C-terminal region of human PHO3.

Isotype: Ig

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This PHO3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 307~337 amino acids from the C-terminal region of human PHO3.

Molecular Weight: 28173 DA

Comments:

Background: PHO3 is a member of the cytidine deaminase gene family. The PHO3 gene is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting: 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Cycle, Signaling, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of hPHO3-D314 (ABIN388145) in mouse testis tissue lysates (35ug/lane). PHO3 (arrow) was detected using the purified Pab.

Publications

Product: Jarmuz, Chester, Bayliss et al.: "An anthropoid-specific locus of orphan C to U RNA-editing enzymes on chromosome 22." in: Genomics, Vol. 79, Issue 3, pp. 285-96, 2002 (PubMed).

Wedekind, Dance, Sowden et al.: "Messenger RNA editing in mammals: new members of the APOBEC family seeking roles in the family business." in: Trends in genetics : TIG, Vol. 19, Issue 4, pp. 207-16, 2003 (PubMed).