Bone Morphogenetic Protein 7 (BMP7) (N-Term) antibody

Antigen: Bone Morphogenetic Protein 7 (BMP7)

Synonyms: OP-1, OP1, bmp7, fb83f09, wu:fb83f09, BMP-7, MGC68434, MGC68426, MGC130772, BMP7, Bmp7, zgc:153025

Epitope: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388457

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: Bmp7 (N-term)

Gene ID: 655

Swiss-Prot: NP_001710

Immunogen: This Bmp7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 22~52 amino acids from the N-terminal region of human Bmp7.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: Bone morphogenetic protein 7 [Precursor], BMP-7, Osteogenic protein 1, OP-1, Eptotermin alfa, BMP7, OP1

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human Bmp7.

Molecular Weight: 49313 DA

Application Details

Application Notes: The suggested dilution will be: ELISA~~1:1,000 Western Blot ~~ 1:50~200 Immunohistochemistry~~1:50~100 Immunofluorescence ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Cell Cycle, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of Bmp7 (arrow) using rabbit polyclonal Bmp7 Antibody (N-term) (ABIN388457). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Bmp7 gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Immunofluorescence analysis of Bmp7 Antibody (N-term) (ABIN388457) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.