NCAPH2 antibody (N-Term)

Catalog No. ABIN388692

Cited in 2+ publications. The Rabbit Polyclonal anti-NCAPH2 antibody (Clone RB9657) (ABIN388692) specifically detects NCAPH2 in WB. The antibody is reactive with Human samples.

Quick Overview for NCAPH2 antibody (N-Term) (ABIN388692)

Target: NCAPH2 (Non-SMC Condensin II Complex, Subunit H2 (NCAPH2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCAPH2 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: RB9657

Product Details anti-NCAPH2 Antibody

Binding Specificity: AA 198-227, N-Term

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This NCAPH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 198-227 amino acids from the N-terminal region of human NCAPH2.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-NCAPH2 antibody (ABIN388692)

Lai, Wong, Lee, Li: "Caspase-3-mediated degradation of condensin Cap-H regulates mitotic cell death." in: Cell death and differentiation, Vol. 18, Issue 6, pp. 996-1004, (2011) (PubMed).

Lisková, Susor, Pivonková, Sasková, Karabínová, Kubelka: "Detection of condensin I and II in maturing pig oocytes." in: Reproduction, fertility, and development, Vol. 22, Issue 4, pp. 644-52, (2010) (PubMed).

Target Details for NCAPH2

Target: NCAPH2 (Non-SMC Condensin II Complex, Subunit H2 (NCAPH2))

Alternative Name: NCAPH2

Background: Structural maintenance of chromosomes (SMC) and non-SMC condensin proteins associate into complexes that have been implicated in the process of chromosome condensation. A crucial prerequisite for accurate segregation of replicated sister chromatids is the condensation of the chromosomes into a manageable form prior to metaphase. The condensin I complex consists of two SMC subunits, SMC2 and SMC4, and three non-SMC subunits, CAP-H, CAP-G, and CAP-D2. An alternative complex, the condensin II complex, contains alternate non-SMC subunits, CAP-G2, CAP-H2, and CAP-D3. CAP-H2 is also known as Non-SMC condensin II complex, subunit H2 (NCAPH2) or kleisin beta isoform 2. The three non-SMC subunits in the condensing complexes form a regulatory subcomplex that is required to activate the SMC ATPases and to promote mitosis-specific chromatin binding of the holocomplex. The precise individual functions of each non-SMC protein in activation remain to be determined.

Molecular Weight: 68227

Gene ID: 29781

NCBI Accession: NP_001171940, NP_055366, NP_689512

UniProt: Q6IBW4